Literature DB >> 4552892

Simple method for the preparation of streptococcal nucleases.

S C Marker, E D Gray.   

Abstract

A simple method for preparation and fractionation of the streptococcal nucleases by polyacrylamide gel electrophoresis is presented. The procedure is carried out with ammonium sulfate-precipitated supernatant fluids from cultures of beta-hemolytic streptococci grown to stationary phase. Electrophoresis on polyacrylamide gel and subsequent elution of the fractionated enzymes allows the preparation of sufficient homogeneous nuclease B for a large number of anti-nuclease B titrations.

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Year:  1972        PMID： 4552892      PMCID： PMC380346          DOI： 10.1128/am.23.2.368-371.1972

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  4 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  The paradox of the antibody response to streptodornase: the usefulness of antidesoxyribonuclease B as an indication of streptococcal infection in patients with acute rheumatic fever.

Authors:  L W WANNAMAKER
Journal:  Am J Med       Date:  1959-10       Impact factor: 4.965

3.  Streptococcal anti-desoxyribonuclease B: microtechnique determination.

Authors:  J Nelson; E M Ayoub; L W Wannamaker
Journal:  J Lab Clin Med       Date:  1968-05

4.  The influence of the site of infection on the immune response to group A streptococci.

Authors:  E L Kaplan; B F Anthony; S S Chapman; E M Ayoub; L W Wannamaker
Journal:  J Clin Invest       Date:  1970-07       Impact factor: 14.808
  4 in total
  12 in total

1.  Isolation of streptococcal nuclease B by batch adsorption.

Authors:  T F Slechta; E D Gray
Journal:  J Clin Microbiol       Date:  1975-12       Impact factor: 5.948

2.  Nuclease content of group H Streptococcus strain Challis cell surface extracts inactivating transforming deoxyribonucleic acid.

Authors:  B J Starościak; P Ceglowski; W T Dobrzański
Journal:  J Bacteriol       Date:  1975-07       Impact factor: 3.490

3.  Enchancement of streptococcal transformation yield by proteolytic enzymes.

Authors:  P G Fuchs; W T Dobrzański
Journal:  J Bacteriol       Date:  1978-10       Impact factor: 3.490

4.  Effect of streptolysin S on human and mouse T and B lymphocytes.

Authors:  W Hryniewicz; J Pryjma
Journal:  Infect Immun       Date:  1977-06       Impact factor: 3.441

5.  Isolation and characterization of cell-associated nucleases related to streptococcal extracellular deoxyribonuclease D.

Authors:  B P Citak; E D Gray
Journal:  J Bacteriol       Date:  1980-02       Impact factor: 3.490

6.  Insertional inactivation of streptolysin S expression in Streptococcus pyogenes.

Authors:  K Nida; P P Cleary
Journal:  J Bacteriol       Date:  1983-09       Impact factor: 3.490

7.  Purification and properties of deoxyribonucleic acid binding factor isolated from the surface of Streptococcus sanguis cells.

Authors:  P Cegłowski; M Kawczyński; W T Dobrzański
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

8.  Towards a better diagnosis of throat infections (with group A beta-haemolytic streptococcus) in general practice.

Authors:  C F Dagnelie; M L Bartelink; Y van der Graaf; W Goessens; R A de Melker
Journal:  Br J Gen Pract       Date:  1998-02       Impact factor: 5.386

9.  Comparison of the titres of ASO, anti-DNase B, and antibodies against the group polysaccharide of group A streptococci in children with streptococcal infections.

Authors:  L E Goedvok-de Groot; N Michel-Bensink; M M van Es-Boon; A H van Vonno; M F Michel
Journal:  J Clin Pathol       Date:  1974-11       Impact factor: 3.411

10.  Biochemical and immunological characterization of the extracellular nucleases of group B streptococci.

Authors:  P Ferrieri; E D Gray; L W Wannamaker
Journal:  J Exp Med       Date:  1980-01-01       Impact factor: 14.307
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