Literature DB >> 454602

A rapid method for the isolation of kidney brush border membranes.

P Malathi, H Preiser, P Fairclough, P Mallett, R K Crane.   

Abstract

A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes.

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Year:  1979        PMID: 454602     DOI: 10.1016/0005-2736(79)90023-3

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  28 in total

1.  The nature of the neutral Na(+)-Cl- coupled entry at the apical membrane of rabbit gallbladder epithelium: III. Analysis of transports on membrane vesicles.

Authors:  G Meyer; G Bottà; C Rossetti; D Cremaschi
Journal:  J Membr Biol       Date:  1990-11       Impact factor: 1.843

2.  The light subunit of system b(o,+) is fully functional in the absence of the heavy subunit.

Authors:  Núria Reig; Josep Chillarón; Paola Bartoccioni; Esperanza Fernández; Annie Bendahan; Antonio Zorzano; Baruch Kanner; Manuel Palacín; Joan Bertran
Journal:  EMBO J       Date:  2002-09-16       Impact factor: 11.598

3.  Specificity and cross-reactive idiotypes of anti-gp330 autoantibodies in active Heymann nephritis.

Authors:  R J Specht Grijp-Glandorf; E De Heer; C C Dekker-Nooren; M R Daha; L A Van Es
Journal:  Immunology       Date:  1990-07       Impact factor: 7.397

4.  Improved diabetic syndrome in C57BL/KsJ-db/db mice by oral administration of the Na(+)-glucose cotransporter inhibitor T-1095.

Authors:  K Arakawa; T Ishihara; A Oku; M Nawano; K Ueta; K Kitamura; M Matsumoto; A Saito
Journal:  Br J Pharmacol       Date:  2001-01       Impact factor: 8.739

5.  Identification of a 400-kd protein in the brush borders of human kidney tubules that is similar to gp330, the nephritogenic antigen of rat Heymann nephritis.

Authors:  D Kerjaschki; R Horvat; S Binder; M Susani; G Dekan; P P Ojha; P Hillemanns; W Ulrich; U Donini
Journal:  Am J Pathol       Date:  1987-10       Impact factor: 4.307

6.  L-glutamate transport in renal plasma membrane vesicles.

Authors:  B Sacktor
Journal:  Mol Cell Biochem       Date:  1981-09-25       Impact factor: 3.396

7.  Identification of high molecular weight antigens structurally related to gamma-glutamyl transferase in epithelial tissues.

Authors:  J D Castle; R S Cameron; P L Patterson; A K Ma
Journal:  J Membr Biol       Date:  1985       Impact factor: 1.843

8.  Epitope specificity of anti-gp330 autoantibodies determines the development of proteinuria in active Heymann nephritis.

Authors:  E H Van Leer; P Ronco; P Verroust; A M van der Wal; P J Hoedemaeker; E De Heer
Journal:  Am J Pathol       Date:  1993-03       Impact factor: 4.307

9.  Specificity of the transport system for tricarboxylic acid cycle intermediates in renal brush borders.

Authors:  S H Wright; I Kippen; J R Klinenberg; E M Wright
Journal:  J Membr Biol       Date:  1980-11-15       Impact factor: 1.843

10.  Antibodies to glycolipids activate complement and promote proteinuria in passive Heymann nephritis.

Authors:  M Susani; M Schulze; M Exner; D Kerjaschki
Journal:  Am J Pathol       Date:  1994-04       Impact factor: 4.307

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