| Literature DB >> 454602 |
P Malathi, H Preiser, P Fairclough, P Mallett, R K Crane.
Abstract
A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes.Entities:
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Year: 1979 PMID: 454602 DOI: 10.1016/0005-2736(79)90023-3
Source DB: PubMed Journal: Biochim Biophys Acta ISSN: 0006-3002