Literature DB >> 454371

Effect of drugs on deoxyribonucleic acid synthesis in isolated mammalian cell nuclei. Comparison with partially purified deoxyribonucleic acid polymerases.

J F Burke, P M Duff, C K Pearson.   

Abstract

In order to ascertain the identity of the DNA-dependent DNA polymerase responsible for the observed DNA synthesis in nuclei isolated from baby-hamster kidney (BHK-21/C13) cells a comparative study was carried out on the effects of some drugs, reported to influence DNA synthesis, on DNA synthesis catalysed by these nuclei and by partially purified DNA polymerase-alpha and -beta. In all cases DNA synthesis by isolated nuclei and polymerase-alpha was inhibited to similar extents by N-ethylmaleimide, p-hydroxymercuribenzoate, novobiocin, heparin and phosphonoacetic acid; polymerase-beta was much less affected by these compounds. Ethidium bromide inhibited all DNA synthesis to similar extents, although at low concentrations (about 2 microgram/ml) synthesis in isolated nuclei was stimulated. The results are discussed in relation to the proposal that DNA polymerase-alpha catalyses the covalent extension of Okazaki fragments that these nuclei carry out in vitro.

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Year:  1979        PMID： 454371      PMCID： PMC1186562          DOI： 10.1042/bj1780621

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  31 in total

1.  Deoxyribonucleic acid polymerases of BHK-21/C13 cells. Partial purification and characterization of the enzymes.

Authors:  R K Craig; H M Keir
Journal:  Biochem J       Date:  1975-02       Impact factor: 3.857

2.  Differentiation and characterization of the cytoplasmic and nuclear deoxyribonucleic acid polymerases from baby hamster kidney cells.

Authors:  L H Lazarus; N Kitron
Journal:  Biochim Biophys Acta       Date:  1975-09-01

3.  Nuclear deoxyribonucleic acid polymerases of liver.

Authors:  W E Lynch; S Surrey; I Lieberman
Journal:  J Biol Chem       Date:  1975-10-25       Impact factor: 5.157

4.  Novikoff hepatoma deoxyribonucleic acid polymerase. Sensitivity of the beta-polymerase to sulfhydryl blocking agents.

Authors:  D W Mosbaugh; T A Kunkel; D M Stalker; J E Tcheng; R R Meyer
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

5.  Involvement of deoxyribonucleic acid polymerase beta in nuclear deoxyribonucleic acid synthesis.

Authors:  T R Butt; W M Wood; E L McKay; R L Adams
Journal:  Biochem J       Date:  1978-07-01       Impact factor: 3.857

6.  Relaxation of chromatin structure induced by ethidium binding: 1--Micrococcal nuclease digestion of the ethidium--chromatin complex.

Authors:  J Paoletti
Journal:  Biochem Biophys Res Commun       Date:  1978-03-15       Impact factor: 3.575

7.  Synthesis of herpes simplex virus, vaccinia virus, and adenovirus DNA in isolated HeLa cell nuclei. I. Effect of viral-specific antisera and phosphonoacetic acid.

Authors:  A Bolden; J Aucker; A Weissbach
Journal:  J Virol       Date:  1975-12       Impact factor: 5.103

8.  Deoxyribonucleic acid synthesis in isolated nuclei from baby-hamster kidney cells (BHK-21/C13). Characterization of the system.

Authors:  J F Burke; C K Pearson
Journal:  Biochem J       Date:  1979-03-15       Impact factor: 3.857

9.  Mechanism of phosphonoacetate inhibition of herpesvirus-induced DNA polymerase.

Authors:  S S Leinbach; J M Reno; L F Lee; A F Isbell; J A Boezi
Journal:  Biochemistry       Date:  1976-01-27       Impact factor: 3.162

10.  DNA polymerases from Chlamydomonas reinhardii. Further characterization, action of inhibitors and associated nuclease activities.

Authors:  C A Ross; W J Harris
Journal:  Biochem J       Date:  1978-04-01       Impact factor: 3.857
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  5 in total

Review 1.  Methods to validate Hsp90 inhibitor specificity, to identify off-target effects, and to rethink approaches for further clinical development.

Authors:  Len Neckers; Brian Blagg; Timothy Haystead; Jane B Trepel; Luke Whitesell; Didier Picard
Journal:  Cell Stress Chaperones       Date:  2018-02-01       Impact factor: 3.667

2.  Does hydroxyurea inhibit DNA replication in mouse cells by more than one mechanism?

Authors:  E Wawra; E Wintersberger
Journal:  Mol Cell Biol       Date:  1983-03       Impact factor: 4.272

3.  Inhibitors of repair DNA synthesis.

Authors:  A R Collins; S Squires; R T Johnson
Journal:  Nucleic Acids Res       Date:  1982-02-25       Impact factor: 16.971

4.  Effect of 4-quinolones and novobiocin on calf thymus DNA polymerase alpha primase complex, topoisomerases I and II, and growth of mammalian lymphoblasts.

Authors:  P Hussy; G Maass; B Tümmler; F Grosse; U Schomburg
Journal:  Antimicrob Agents Chemother       Date:  1986-06       Impact factor: 5.191

5.  Replication initiation complex formation in the absence of nuclear function in Xenopus.

Authors:  Liliana Krasinska; Daniel Fisher
Journal:  Nucleic Acids Res       Date:  2009-02-22       Impact factor: 16.971
  5 in total

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