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Literature DB >> 4526223

Chemical synthesis of a primer and its use in the sequence analysis of the lysozyme gene of bacteriophage T4.

Abstract

We have developed a general approach for determining the nucleotide sequence of a gene, with the aid of a deoxyribonucleotide primer of defined sequence. The selection of the primer sequence was based on a short segment of mRNA sequence of T4 phage lysozyme. A tetradecadeoxyribonucleotide primer was chemically synthesized and its sequence verified by sequence analysis. This primer was found to bind to the single-stranded region of the exonuclease III-treated T4 DNA, and specific nucleotides were incorporated to its 3' end. The result indicated that this primer was bound to the expected location on the T4 DNA. Therefore, long sequences of the T4 lysozyme gene can now be determined from this specific starting point.

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Year: 1974 PMID： 4526223 PMCID： PMC388489 DOI： 10.1073/pnas.71.6.2510

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. Nucleotide sequence analysis of DNA. IX. Use of oligonucleotides of defined sequence as primers in DNA sequence analysis.

Authors: R Padmanabhan; R Wu
Journal: Biochem Biophys Res Commun Date: 1972-09-05 Impact factor: 3.575

9. CXI. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Synthesis of a dodecadeoxynucleotide and a heptadeoxynucleotide corresponding to the nucleotide sequence 66 to 77.

Authors: M H Caruthers; H G Khorana
Journal: J Mol Biol Date: 1972-12-28 Impact factor: 5.469

10. Studies on polynucleotides. 98. A convenient and general method for the preparation of protected dideoxyribonucleotides containing 5'-phosphate end groups.

Authors: K L Agarwal; A Yamazaki; H G Khorana
Journal: J Am Chem Soc Date: 1971-06-02 Impact factor: 15.419

1 in total

Review 1. The sequence of sequencers: The history of sequencing DNA.

Authors: James M Heather; Benjamin Chain
Journal: Genomics Date: 2015-11-10 Impact factor: 5.736

1 in total

Chemical synthesis of a primer and its use in the sequence analysis of the lysozyme gene of bacteriophage T4.

1. Nucleotide sequence analysis of DNA. IX. Use of oligonucleotides of defined sequence as primers in DNA sequence analysis.

2. Nucleotide sequence analysis of DNA.

3. A new method for the sequence analysis of oligodeoxynucleotides.

4. Nucleotide sequence analysis of DNA. I. Partial sequence of the cohesive ends of bacteriophage lambda and 186 DNA.

5. Complete primary structure of phage lysozyme from Escherichia coli T4.

6. Identification of the transcribing DNA strand for the rII and endolysin genes of coliphage T4.

7. Chemical synthesis of two deoxyribopolynucleotide fragments containing the natural sequence of T4 lysozyme gene e.

8. Chromatography of nucleic acid digests on thin layers of cellulose impregnated with polyethyleneimine.

9. CXI. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Synthesis of a dodecadeoxynucleotide and a heptadeoxynucleotide corresponding to the nucleotide sequence 66 to 77.

10. Studies on polynucleotides. 98. A convenient and general method for the preparation of protected dideoxyribonucleotides containing 5'-phosphate end groups.

Review 1. The sequence of sequencers: The history of sequencing DNA.