Literature DB >> 4526218

Enhancement of DNA synthesis in a mammalian cell-free system by trypsin treatment.

R L Brown, E Stubblefield.   

Abstract

DNA synthesis in a broken cellular preparation of Chinese hamster cells was enhanced approximately 10-fold by a brief trypsin treatment. Alphachymotrypsin also enhanced the synthesis, whereas Pronase did not. The trypsin appears to be acting on a component of the nucleus. Evidence suggests that the trypsin is not removing protein from the DNA, but may be activating the system some other way.

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Year:  1974        PMID： 4526218      PMCID： PMC388471          DOI： 10.1073/pnas.71.6.2432

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  Accessibility of DNA in chromatin to DNA polymerase and RNA polymerase.

Authors:  B Silverman; A E Mirsky
Journal:  Proc Natl Acad Sci U S A       Date:  1973-05       Impact factor: 11.205

2.  Replication of DNA in mammalian chromosomes. II. Kinetics of 3H-thymidine incorporation and the isolation and partial characterization of labeled subunits at the growing point.

Authors:  J H Taylor; A G Adams; M P Kurek
Journal:  Chromosoma       Date:  1973-04-27       Impact factor: 4.316

3.  DNA methylation in isolated nuclei: old and new DNAs are methylated.

Authors:  R L Adams; C Hogarth
Journal:  Biochim Biophys Acta       Date:  1973-12-07

4.  The presence of a common active subunit in low and high molecular weight murine DNA polymerases.

Authors:  N B Hecht; D Davidson
Journal:  Biochem Biophys Res Commun       Date:  1973-03-17       Impact factor: 3.575

5.  RNA bound to nascent DNA in Ehrlich ascites tumor cells.

Authors:  S Sato; S Ariake; M Saito; T Sugimura
Journal:  Biochem Biophys Res Commun       Date:  1972-11-01       Impact factor: 3.575

6.  A fluorometric method for the detection of endodeoxyribonuclease on DNA-polyacrylamide gels.

Authors:  D J Grdina; P H Lohman; R R Hewitt
Journal:  Anal Biochem       Date:  1973-01       Impact factor: 3.365

7.  Effect of trypsin on DNA methylation in isolated nuclei from developing sea urchin embryos.

Authors:  L Tosi; E Scarano
Journal:  Biochem Biophys Res Commun       Date:  1973-11-16       Impact factor: 3.575

8.  Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow.

Authors:  L M Chang; F J Bollum
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

9.  Sea urchin nuclear DNA polymerase. II. Changing localization during early development.

Authors:  B Fansler; L A Loeb
Journal:  Exp Cell Res       Date:  1969-10       Impact factor: 3.905

10.  A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH.

Authors:  W Wray; E Stubblefield
Journal:  Exp Cell Res       Date:  1970-03       Impact factor: 3.905
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  4 in total

1.  Influence of adeno-associated virus on adherence and growth properties of normal cells.

Authors:  U Bantel-Schaal; M Stöhr
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

2.  Transient recovery from trypsin-caused reduction in fertilizability of sea urchin eggs.

Authors:  G Czihak; K Aketa; H Vogel; P Chang; W Wolf
Journal:  Wilehm Roux Arch Dev Biol       Date:  1982-05

3.  Induction of DNA synthesis in isolated nuclei by cytoplasmic factors: inhibition by protease inhibitors.

Authors:  R L Wong; J K Gutowski; M Katz; R H Goldfarb; S Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1987-01       Impact factor: 11.205

4.  Deoxyribonucleic acid synthesis in isolated nuclei from baby-hamster kidney cells (BHK-21/C13). Characterization of the system.

Authors:  J F Burke; C K Pearson
Journal:  Biochem J       Date:  1979-03-15       Impact factor: 3.857
  4 in total

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