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Literature DB >> 4525471

Reconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B.

G Veomett, D M Prescott, J Shay, K R Porter.

Abstract

Mouse L929 cells were separated into enucleated cytoplasmic components (cytoplasts) and nucleated subcellular fractions (karyoplasts) in the presence of cytochalasin B. Karyoplasts from cells containing tritiated nuclei were fused, using inactivated Sendai virus, to cytoplasts from cells containing large (1.0-mum diameter) latex spheres in the cytoplasm. Mononucleated cells containing radioactive nuclei and large latex spheres in the cytoplasm were observed among the products of the fusion reaction. Some of these cells were in mitotic configurations. The results indicate that cells capable of undergoing mitosis can be reconstructed from the products of cellular enucleation in the presence of cytochalasin B.

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Year: 1974 PMID： 4525471 PMCID： PMC388372 DOI： 10.1073/pnas.71.5.1999

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

6 in total

1. Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B.

Authors: R Pollack; R Goldman
Journal: Science Date: 1973-03-02 Impact factor: 47.728

2. Ultrastructure of enucleated mammalian cells in culture.

Authors: G E Wise; D M Prescott
Journal: Exp Cell Res Date: 1973-09 Impact factor: 3.905

3. Preservation of normal behavior by enucleated cells in culture.

Authors: R D Goldman; R Pollack; N H Hopkins
Journal: Proc Natl Acad Sci U S A Date: 1973-03 Impact factor: 11.205

4. Enucleation of mammalian cells with cytochalasin B.

Authors: D M Prescott; D Myerson; J Wallace
Journal: Exp Cell Res Date: 1972 Impact factor: 3.905

5. Replication of vaccinia virus DNA in enucleated L-cells.

Authors: D M Prescott; J Kates; J B Kirkpatrick
Journal: J Mol Biol Date: 1971-08-14 Impact factor: 5.469

6. Effects of cytochalasins on mammalian cells.

Authors: S B Carter
Journal: Nature Date: 1967-01-21 Impact factor: 49.962

6 in total

20 in total

10. Identification of key regulated events early in the life of hybrid animal cells constructed by nuclear transplantation.

Authors: M J Hightower; J Bruno; J J Lucas
Journal: Proc Natl Acad Sci U S A Date: 1983-09 Impact factor: 11.205

Reconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B.

1. Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B.

2. Ultrastructure of enucleated mammalian cells in culture.

3. Preservation of normal behavior by enucleated cells in culture.

4. Enucleation of mammalian cells with cytochalasin B.

5. Replication of vaccinia virus DNA in enucleated L-cells.

6. Effects of cytochalasins on mammalian cells.

Review 1. Microcell-mediated chromosome transfer (MMCT): small cells with huge potential.

2. Intracellular localization of mouse DNA polymerase-alpha.

3. Heterokaryons in the analysis of genes and gene regulation.

4. Cell fusion and plasticity.

5. Cyclic AMP, the microtubule-microfilament system, and cancer.

6. Enucleation and reconstruction of interferon-producing cells.

7. Late S phase cells (Chinese hamster ovary) induce early S phase DNA labeling patterns in G1 phase nuclei.

8. Tumorigenicity and its suppression in cybrids of mouse and Chinese hamster cell lines.

9. Selection of reconstituted cells from karyoplasts fused to chloramphenicol-resistant cytoplasts.

10. Identification of key regulated events early in the life of hybrid animal cells constructed by nuclear transplantation.