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Literature DB >> 4519655

Molecular nature of mammalian cell DNA in alkaline sucrose gradients.

J R Simpson, W A Nagle, M D Bick, J A Belli.

Abstract

Mammalian cell DNA that exhibited anomalous sedimentation in alkaline sucrose gradients was examined directly by electron microscopy. Its appearance was that of duplex DNA. In addition, some duplex DNA was observed under conditions in which the sedimentation anomaly was no longer apparent. Persistence of double-stranded DNA under denaturing conditions suggests caution in the interpretation of changes in the molecular weight or conformation of DNA based solely on analysis of sedimentation profiles.

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Year: 1973 PMID： 4519655 PMCID： PMC427301 DOI： 10.1073/pnas.70.12.3660

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

Molecular nature of mammalian cell DNA in alkaline sucrose gradients.

1. Characterization of a naturally occurring, cross-linked fraction of DNA. 1. Nature of the cross-linkage.

2. Characterization of a naturally occurring, cross-linked fraction of DNA. II. Origin of the cross-linkage.

3. Interstrand cross-linking of DNA by difunctional alkylating agents.

4. The rationale for an ordered arrangement of chromatin in the interphase nucleus.

5. DNA fibres from human lymphocyte nuclei.

6. Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces.

7. Artefact in the measurement of the molecular weight of pulse labelled DNA.

8. Radiation-induced breaks of DNA in cultured mammalian cells.

9. Mechanism of inactivation of DNA and RNA bacteriophages by alkylating agents in vitro.

10. Rejoining of x-ray induced breaks in the DNA of leukaemia cells.

1. Viscoelastic analysis of high molecular weight, alkali-denatured DNA from mouse 3T3 cells.

2. Single strand breakage and repair in eukaryotic DNA as assayed by S1 nuclease.

3. Effect of alkali on the size dispersity of mammalian DNA measured by filter elution.

4. A mechanistic basis for amplification differences between samples and between genome regions.