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Literature DB >> 4519642

Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA.

R B Goldberg, G A Galau, R J Britten, E H Davidson.

Abstract

Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusively with nonrepetitive DNA. Almost all of the messenger RNA molecules of sea urchin gastrulae therefore consist of transcripts from nonrepetitive sequences. It appears that the structural genes expressed at this stage are typically not repeated in the genome and the mRNA does not include recognizable repetitive sequence.

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Year: 1973 PMID： 4519642 PMCID： PMC427271 DOI： 10.1073/pnas.70.12.3516

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

10. Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase.

Authors: B Levy; G H Dixon
Journal: Nucleic Acids Res Date: 1977-04 Impact factor: 16.971

Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA.

1. DNA-RNA hybridization in vast DNA excess.

2. Isolation of chromatin bearing nascent RNA from nuclei of sea urchin embryos.

3. Measurements of messenger RNA entering polysomes upon fertilization of sea urchin eggs.

4. Synthesis of nuclear and chromosomal proteins on light polyribosomes during cleavage in the sea urchin embryo.

5. Gene regulation for higher cells: a theory.

6. Reiteration and clustering of DNA sequences complementary to histone messenger RNA.

7. Release, identification, and isolation of messenger RNA from mammalian ribosomes.

8. Characterization of macromolecules by constant velocity sedimentation.

9. Transcription of nonrepeated DNA in neonatal and fetal mice.

10. Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA.

1. Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

2. Profile of Robert B. Goldberg.

3. Evolutionary divergence and length of repetitive sequences in sea urchin DNA.

4. Transfer of duck cell DNA sequences to the nucleus of 3T3 cells by Rous sarcoma virus.

5. Conservation of repeated DNA base sequences in Crustacea: a molecular approach to decapod phylogeny.

6. Sequence diversity of polysomal mRNAs in roots and shoots of etiolated and greened pea seedlings.

7. Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms.

8. A genetic map of citrus based on the segregation of isozymes and RFLPs in an intergeneric cross.

9. Messenger RNA abundance and lifetime: a correlation in Drosophila cells but not in HeLa.

10. Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase.