Literature DB >> 4517943

Nuclear magnetic resonance study of hydrogen-bonded ring protons in Watson-Crick base pairs.

D M Crothers, C W Hilbers, R G Shulman.   

Abstract

We have studied the low-field proton magnetic resonance spectrum of the double-helical complex of d(A-A-C-A-A) with d(T-T-G-T-T). Well-resolved resonances for the thymidine N(3) and the guanosine N(1) protons are seen at 1 degrees C, which broaden by 4 degrees C and disappear above 9 degrees C. The actual T(m) of the complex was found to be 28 degrees C by measurement of UV-absorbance change. There are several factors that could limit the observed exchange broadening of the resonance lines; the model that best fits the data is one in which the rate of double-helix dissociation limits the rate of exchange of the H-bonding protons with water.

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Year:  1973        PMID： 4517943      PMCID： PMC427134          DOI： 10.1073/pnas.70.10.2899

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Conformational changes of transfer ribonucleic acid. Comparison of the early melting transition of two tyrosine-specific transfer ribonucleic acids.

Authors:  S K Yang; D M Crothers
Journal:  Biochemistry       Date:  1972-11-07       Impact factor: 3.162

2.  High resolution nuclear magnetic resonance studies of hydrogen bonded protons of tRNA in water.

Authors:  D R Kearns; D J Patel; R G Shulman
Journal:  Nature       Date:  1971-01-29       Impact factor: 49.962

3.  Relaxation kinetics of dimer formation by self complementary oligonucleotides.

Authors:  M E Craig; D M Crothers; P Doty
Journal:  J Mol Biol       Date:  1971-12-14       Impact factor: 5.469

4.  A proton magnetic resonance study of single-stranded and double-helical deoxyribooligonucleotides.

Authors:  A D Cross; D M Crothers
Journal:  Biochemistry       Date:  1971-10-26       Impact factor: 3.162

5.  Intermolecular nuclear shielding values for protons of purines and flavins.

Authors:  C Giessner-Prettre; B Pullman
Journal:  J Theor Biol       Date:  1970-04       Impact factor: 2.691

6.  Hydrogen exchange as a probe of the dynamic structure of DNA. I. General acid-base catalysis.

Authors:  B McConnell; P H von Hippel
Journal:  J Mol Biol       Date:  1970-06-14       Impact factor: 5.469

7.  Nuclear magnetic resonance study of cyanoferrimyoglobin; identification of pseudocontact shifts.

Authors:  B Sheard; T Yamane; R G Shulman
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

8.  Association by hydrogen bonding of free nucleosides in non-aqueous solution.

Authors:  L Katz; S Penman
Journal:  J Mol Biol       Date:  1966-01       Impact factor: 5.469

9.  NMR evidence of specific base-pairing between purines and pyrimidines.

Authors:  R R Shoup; H T Miles; E D Becker
Journal:  Biochem Biophys Res Commun       Date:  1966-04-19       Impact factor: 3.575

10.  Co-operative non-enzymic base recognition. 3. Kinetics of the helix-coil transition of the oligoribouridylic--oligoriboadenylic acid system and of oligoriboadenylic acid alone at acidic pH.

Authors:  D Pörschke; M Eigen
Journal:  J Mol Biol       Date:  1971-12-14       Impact factor: 5.469
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  8 in total

1.  High-resolution proton magnetic resonance study of the secondary structure of the 3'-terminal 49-nucleotide fragment of 16S rRNA from Escherichia coli.

Authors:  R A Baan; C W Hilbers; R Van Charldorp; E Van Leerdam; P H Van Knippenberg; L Bosch
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

2.  1H NMR investigation of the conformational states of DNA in nucleosome core particles.

Authors:  J Feigon; D R Kearns
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

3.  Steroid diamine-nucleic acid interactions: partial insertion of dipyrandium between unstacked base pairs of the poly(dA-dT) duplex in solution.

Authors:  D J Patel; L L Canuel
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

4.  Sequence dependence of hydrogen exchange kinetics in DNA duplexes at the individual base pair level in solution.

Authors:  D J Patel; S Ikuta; S Kozlowski; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1983-04       Impact factor: 11.205

5.  Physical studies of denatured tRNA2Glu from Escherichia coli.

Authors:  M Bina-Stein; D M Crothers; C W Hilbers; R G Shulman
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

6.  A historical account of Hoogsteen base-pairs in duplex DNA.

Authors:  Evgenia N Nikolova; Huiqing Zhou; Federico L Gottardo; Heidi S Alvey; Isaac J Kimsey; Hashim M Al-Hashimi
Journal:  Biopolymers       Date:  2013-12       Impact factor: 2.505

7.  Single-molecule kinetics reveal microscopic mechanism by which High-Mobility Group B proteins alter DNA flexibility.

Authors:  Micah J McCauley; Emily M Rueter; Ioulia Rouzina; L James Maher; Mark C Williams
Journal:  Nucleic Acids Res       Date:  2012-11-09       Impact factor: 16.971

8.  The impact of hydrogen bonding on amide 1H chemical shift anisotropy studied by cross-correlated relaxation and liquid crystal NMR spectroscopy.

Authors:  Lishan Yao; Alexander Grishaev; Gabriel Cornilescu; Ad Bax
Journal:  J Am Chem Soc       Date:  2010-08-11       Impact factor: 15.419
  8 in total

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