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Literature DB >> 4506784

Regeneration of cation-transport capacity in HeLa cell membranes after specific blockade by ouabain.

Abstract

The cardiac glycoside, ouabain, inhibits alkali-cation transport in HeLa cells. It binds to 0.75 x 10(6) sites per cell, and the half-time for its dissociation is 16 hr. After partial blockade by ouabain, the cell generates new ouabain-binding sites, with total restoration of transport in 10% of a cell cycle( approximately 3 hr). This recovery requires protein synthesis and appears to be a response to altered cell-electrolyte content, since growth of cells in media with low K(+) concentration enhances the titer of the transport enzyme in a fashion similar to the effect of ouabain. Totally blocked cells do not recover.

Entities: Chemical

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Year: 1972 PMID： 4506784 PMCID： PMC427003 DOI： 10.1073/pnas.69.9.2627

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. Regeneration of transplantation antigens on mouse cells.

Authors: B D Schwartz; S G Nathenson
Journal: Transplant Proc Date: 1971-03 Impact factor: 1.066

2. Potassium ions and the binding of cardiac glycosides to mammalian cells.

Authors: P F Baker; J S Willis
Journal: Nature Date: 1970-05-09 Impact factor: 49.962

3. Monovalent cation requirement for the aminoacyl transfer reaction in protein synthesis.

Authors: H Levine; M R Trindle; K Moldave
Journal: Nature Date: 1966-09-17 Impact factor: 49.962

4. [On the influence of radiation-induced electropyte displacements on metabolism. I. Studies on acetyl-CoA synthetase in yeast].

Authors: H Rink; H D Bergeder
Journal: Strahlentherapie Date: 1968-03

5. On the number of sodium pumping sites in cell membranes.

Authors: P F Baker; J S Willis
Journal: Biochim Biophys Acta Date: 1969

6. Uptake of (3H)ouabain and Na pump turnover rates in monolayer cultures of Girardi heart cells.

Authors: J F Lamb; D McCall
Journal: J Physiol Date: 1971-03 Impact factor: 5.182

7. Virus-receptor interaction in an adenovirus system.

Authors: L Philipson; K Lonberg-Holm; U Pettersson
Journal: J Virol Date: 1968-10 Impact factor: 5.103

8. Cation metabolism in relation to cell size in synchronously grown tissue culture cell.

Authors: C Jung; A Rothstein
Journal: J Gen Physiol Date: 1967-03 Impact factor: 4.086

9. Membranes of animal cells. II. The metabolism and turnover of the surface membrane.

Authors: L Warren; M C Glick
Journal: J Cell Biol Date: 1968-06 Impact factor: 10.539

10. Cholesterol metabolism in the macrophage. II. Alteration of subcellular exchangeable cholesterol compartments and exchange in other cell types.

Authors: Z Werb; Z A Cohn
Journal: J Exp Med Date: 1971-12-01 Impact factor: 14.307

19 in total

1. Ultrastructural, cyto- and biochemical observations during turnover of plasma membrane in duck salt gland.

Authors: F E Hossler; M P Sarras; E R Allen
Journal: Cell Tissue Res Date: 1978-04-17 Impact factor: 5.249

2. Chronic digoxin treatment on canine myocardial Na+, K+ -ATPase.

Authors: D D Ku; T Akera; T M Brody; L C Weaver
Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 1977-12 Impact factor: 3.000

3. Differential chemical protection of mammalian cells from the exotoxins of Corynebacterium diphtheriae and Pseudomonas aeruginosa.

Authors: J L Middlebrook; R B Dorland
Journal: Infect Immun Date: 1977-04 Impact factor: 3.441

Regeneration of cation-transport capacity in HeLa cell membranes after specific blockade by ouabain.

1. Regeneration of transplantation antigens on mouse cells.

2. Potassium ions and the binding of cardiac glycosides to mammalian cells.

3. Monovalent cation requirement for the aminoacyl transfer reaction in protein synthesis.

4. [On the influence of radiation-induced electropyte displacements on metabolism. I. Studies on acetyl-CoA synthetase in yeast].

5. On the number of sodium pumping sites in cell membranes.

6. Uptake of (3H)ouabain and Na pump turnover rates in monolayer cultures of Girardi heart cells.

7. Virus-receptor interaction in an adenovirus system.

8. Cation metabolism in relation to cell size in synchronously grown tissue culture cell.

9. Membranes of animal cells. II. The metabolism and turnover of the surface membrane.

10. Cholesterol metabolism in the macrophage. II. Alteration of subcellular exchangeable cholesterol compartments and exchange in other cell types.

1. Ultrastructural, cyto- and biochemical observations during turnover of plasma membrane in duck salt gland.

2. Chronic digoxin treatment on canine myocardial Na+, K+ -ATPase.

3. Differential chemical protection of mammalian cells from the exotoxins of Corynebacterium diphtheriae and Pseudomonas aeruginosa.

4. Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells.

5. The receptor function of the Na+, K+-activated adenosine triphosphatase system.

Review 6. Ion concentration-dependent regulation of Na,K-pump abundance.

7. Ouabain is a reversible inhibitor of myogenic fusion.

8. Effect of ouabain on sodium transport across hormone-stimulated toad bladder and skin.

9. Photochemical damage to skin fibroblasts caused by protoporphyrin and violet light.

10. Developmental changes in metabolism and transport properties of capillaries isolated from rat brain.