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Literature DB >> 4505656

DNA polymerase of chicken embryo: purification and properties.

J G Stavrianopoulos, J D Karkas, E Chargaff.

Abstract

The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn(2+) ion for activity, and may contain Zn. It is very sensitive to oxidation.

Entities: Chemical Species

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Year: 1972 PMID： 4505656 PMCID： PMC426801 DOI： 10.1073/pnas.69.7.1781

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

8 in total

1. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

2. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors: R A REISFELD; U J LEWIS; D E WILLIAMS
Journal: Nature Date: 1962-07-21 Impact factor: 49.962

3. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.

Authors: T M Jovin; P T Englund; L L Bertsch
Journal: J Biol Chem Date: 1969-06-10 Impact factor: 5.157

4. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

5. Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template.

Authors: J G Stavrianopoulos; J D Karkas; E Chargaff
Journal: Proc Natl Acad Sci U S A Date: 1971-09 Impact factor: 11.205

6. Zinc in DNA polymerases.

Authors: J P Slater; A S Mildvan; L A Loeb
Journal: Biochem Biophys Res Commun Date: 1971-07-02 Impact factor: 3.575

7. Doxynucleotide-polymerizing enzymes of calf thymus gland. IV. Inhibition of terminal deoxynucleotidyl transferase by metal ligands.

Authors: L M Chang; F J Bollum
Journal: Proc Natl Acad Sci U S A Date: 1970-04 Impact factor: 11.205

8. Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors: P Andrews
Journal: Biochem J Date: 1964-05 Impact factor: 3.766

8 in total

14 in total

9. Hepatitis-B virus-associated deoxyribonucleic acid polymerase: a partial characterization by the use of chemical agents.

Authors: G Hess; W Arnold; K H Meyer zum Büschenfelde
Journal: Klin Wochenschr Date: 1981-06-15

10. Inhibition of RNA-dependent DNA polymerase of Rous sarcoma virus by thiosemicarbazones and several cations.

Authors: W Levinson; A Faras; B Woodson; J Jackson; J M Bishop
Journal: Proc Natl Acad Sci U S A Date: 1973-01 Impact factor: 11.205

DNA polymerase of chicken embryo: purification and properties.

1. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

2. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

3. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.

4. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

5. Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template.

6. Zinc in DNA polymerases.

7. Doxynucleotide-polymerizing enzymes of calf thymus gland. IV. Inhibition of terminal deoxynucleotidyl transferase by metal ligands.

8. Estimation of the molecular weights of proteins by Sephadex gel-filtration.

1. A low molecular weight DNA polymerase from wheat embryos.

2. Human cytomegalovirus. III. Virus-induced DNA polymerase.

3. Purification and characterization of varicella-zoster virus-induced DNA polymerase.

4. DNA polymerases from Chlamydomonas reinhardii. Purification and properties.

5. Mechanism of DNA replication by highly purified DNA polymerase of chicken embryo.

6. Purification and properties of ribonuclease H of calf thymus.

Review 7. RNA-dependent DNA polymerase (reverse transcriptase).

8. Survey of DNA polymerase activity during the early development of Drosophila melanogaster.

9. Hepatitis-B virus-associated deoxyribonucleic acid polymerase: a partial characterization by the use of chemical agents.

10. Inhibition of RNA-dependent DNA polymerase of Rous sarcoma virus by thiosemicarbazones and several cations.