A simple method of detecting staphylococcal hemolysins.

A modification of the "one-plate method" for detection of staphylococcal hemolysins has been described. Bacterial strains of Staphylococcus aureus, Streptococcus agalactiae and Cornebacterium pseudotuberculosis were replaced by their prepurified hemolytically active exosubstances: beta-toxin, CAMP-factor, and, the exosubstance of Corynebacterium pseudotuberculosis called COREX. By means of the modification described, exact detection of hemolysins in possible with staphylococcal strains producing one type of hemolysin as well as with strains producing a combination of hemolysins.