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Literature DB >> 4476072

The fractionation of calf thymus DNA by multiple formation of sedimentable complexes with homologous lysine-rich histone KAP. Derivative melting curves and ultracentrifugation in CsCl concentration gradients.

A Plucienniczak, J Bartkowiak, A Krzywiec, H Panusz.

Abstract

The process of fractionation of total calf thymus DNA using a step precipitation of DNA by means of increasing concentrations of the homologous histone KAP was investigated. In addition to the known fractions three so far undescribed ones/in thymus/,characterized by buoyant densities in CsCl equal 1.692, 1.706 and 1.728 g/ccm, were identified. Considerable amounts of preparations seriously enriched in individual satellite fractions were obtained. The ability of GC-rich satellite DNAs to form more soluble complexes with histone KAP is suggested as reason for the observed fractionation.

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Year: 1974 PMID： 4476072 PMCID： PMC343447 DOI： 10.1093/nar/1.12.1675

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

18 in total

1. Specificity in the association of histones with deoxyribonucleic acid. Evidence from derivative thermal denaturation profiles.

Authors: A T Ansevin; B W Brown
Journal: Biochemistry Date: 1971-03-30 Impact factor: 3.162

2. Multiple satellite deoxyribonucleic acids in the calf and their relation to the sex chromosomes.

Authors: D M Kurnit; B R Shafit; J J Maio
Journal: J Mol Biol Date: 1973-12-15 Impact factor: 5.469

3. Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA.

Authors: Y V Ilyin; A Y Varshavsky; U N Mickelsaar; G P Georgiev
Journal: Eur J Biochem Date: 1971-09-24

1. Specifity of DNA-basic polypeptide interactions. Influence of neutral residues incorporated into polylysine and polyarginine.

Authors: K Wehling; H A Arfmann; K H Standke; K G Wagner
Journal: Nucleic Acids Res Date: 1975-06 Impact factor: 16.971

Review 2. DNA sequence specific interactions of histone H1.

Authors: J Zlatanova; J Yaneva
Journal: Mol Biol Rep Date: 1991-02 Impact factor: 2.316

2 in total

The fractionation of calf thymus DNA by multiple formation of sedimentable complexes with homologous lysine-rich histone KAP. Derivative melting curves and ultracentrifugation in CsCl concentration gradients.

1. Specificity in the association of histones with deoxyribonucleic acid. Evidence from derivative thermal denaturation profiles.

2. Multiple satellite deoxyribonucleic acids in the calf and their relation to the sex chromosomes.

3. Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA.

4. Satellite DNA in calf heterochromatin.

5. Renaturation properties and localization in heterochromatin of human satellite DNA's.

6. Rapid equilibrium isopycnic CsC1 gradients.

7. [Interaction of deoxyribonucleic acid and histones].

8. Studies on histones. 7. Preparative methods for histone fractions from calf thymus.

9. Renaturation of calf thymus satellite DNA.

10. Satellite sequences in chimpanzee (Pan troglodytes).

1. Specifity of DNA-basic polypeptide interactions. Influence of neutral residues incorporated into polylysine and polyarginine.

Review 2. DNA sequence specific interactions of histone H1.