Literature DB >> 446690

A source of error in the fluorometric determination of protein in human placental microsomes.

C H Blomquist, C E Kotts.   

Abstract

When protein is assayed in human placental microsomes by the fluorescamine procedure, 25--30% of the observed fluorescence results from labeling of phospholipids.

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Year:  1979        PMID： 446690     DOI： 10.1007/bf01960411

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  5 in total

1.  Adaptation of a fluorescent protein assay for use with cell fractions and homogenates.

Authors:  R M Van Frank
Journal:  Anal Biochem       Date:  1975-05-12       Impact factor: 3.365

2.  Use of fluorescamine for the estimation of protein in the presence of detergent Triton X-100.

Authors:  N R Sims; P R Carnegie
Journal:  Anal Biochem       Date:  1975-05-12       Impact factor: 3.365

3.  Fluorometric assay of proteins in the nanogram range.

Authors:  P Böhlen; S Stein; W Dairman; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1973-03       Impact factor: 4.013

4.  A simplified protein assay with fluorescamine in samples containing interfering material.

Authors:  P Böhlen; S Stein; K Imai; S Udenfriend
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

5.  [The phospholipid dependence, enrichment and characterization of microsomal 17beta-hydroxysteroid-oxido reductase and "transhydrogenase activity" from human placenta (author's transl)].

Authors:  K Pollow; W Runge; B Pollow; H Grunz; W R Willems; J Schmalbeck
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1973-07
  5 in total

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