Literature DB >> 446642

The morphology of cytoplasts and karyoplasts produced by cytochalasin D.

L Skinnider.   

Abstract

The denucleation of L cells and human lymphocytes in suspension was carried out by incubation with cytochalasin D25 and 50 microgram/ml and with ultracentrifugation over a discontinuous Ficoll gradient. Ultrastructural examination of the separate layers confirmed the presence of the anucleate cytoplasm (cytoplasts) with intact membrane and the separated nuclei (karyoplasts).

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Year:  1979        PMID: 446642     DOI: 10.1007/bf01964390

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  5 in total

1.  The cellular response to cytochalasin B: a critical overview.

Authors:  M Copeland
Journal:  Cytologia (Tokyo)       Date:  1974-12       Impact factor: 0.791

2.  Preservation of normal behavior by enucleated cells in culture.

Authors:  R D Goldman; R Pollack; N H Hopkins
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

3.  Enucleation of mammalian cells with cytochalasin B.

Authors:  D M Prescott; D Myerson; J Wallace
Journal:  Exp Cell Res       Date:  1972       Impact factor: 3.905

4.  Characterization of minicells (nuclei) obtained by cytochalasin enucleation.

Authors:  T Ege; H Hamberg; U Krondahl; J Ericsson; N R Ringertz
Journal:  Exp Cell Res       Date:  1974-08       Impact factor: 3.905

5.  Effects of cytochalasins on mammalian cells.

Authors:  S B Carter
Journal:  Nature       Date:  1967-01-21       Impact factor: 49.962

  5 in total

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