| Literature DB >> 446642 |
Abstract
The denucleation of L cells and human lymphocytes in suspension was carried out by incubation with cytochalasin D25 and 50 microgram/ml and with ultracentrifugation over a discontinuous Ficoll gradient. Ultrastructural examination of the separate layers confirmed the presence of the anucleate cytoplasm (cytoplasts) with intact membrane and the separated nuclei (karyoplasts).Entities:
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Year: 1979 PMID: 446642 DOI: 10.1007/bf01964390
Source DB: PubMed Journal: Experientia ISSN: 0014-4754