Difference in kinetic properties between hexokinase type I isoenzymes from various rat tissues with reference to the effect of a thiol inhibitor.

Hexokinase isoenzyme type I was purified from various rat tissues, and was subjected to kinetic studies in the presence or the absence of p-chloromercuribenzenesulphonate. The mode of the inhibition by the thiol inhibitor was different for the type I isoenzymes obtained from different tissues, suggesting that the type I isoenzymes from different tissues were not identical proteins.