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Literature DB >> 4455205

Alkaline phosphatase from pig kidney. Method of purification and molecular properties.

Abstract

Alkaline phosphatase (EC 3.1.3.1) from pig kidney brush-border membranes was solubilized from membrane precipitates by butan-1-ol at a critical pH of 7.0. The 12000-fold purification procedure included (NH(4))(2)SO(4) precipitation, DEAE-and TEAE-cellulose chromatography, Sephadex G-200 gel filtration and neuraminidase digestion followed by DEAE-cellulose chromatography. The purified protein contained 20% (w/w) carbohydrate and had mol.wt. 150000-156000 as estimated by Sephadex filtration and ultracentrifuge analysis. It was a tetrameric glycoprotein consisting of identical subunits, and it had a molecular activity at 25 degrees C of 2600s(-1) per tetramer. Its concentration in kidney was estimated to be 8.5-8.8mg/kg.

Entities: Chemical Species

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Year: 1974 PMID： 4455205 PMCID： PMC1168074 DOI： 10.1042/bj1410273

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

35 in total

1. The purification of aklaline phosphatases of animal tissues.

Authors: R K MORTON
Journal: Biochem J Date: 1954-08 Impact factor: 3.857

2. [Morphological and biochemical studies on the surface structure of the rat kidney brush-border membrane].

Authors: H Pockrandt-Hemstedt; J E Schmitz; E Kinne-Saffran; R Kinne
Journal: Pflugers Arch Date: 1972 Impact factor: 3.657

3. [Critical electron microscopical studies on the localization of activity of alkaline phosphatase in the main portion of the kidney of mice].

Authors: E Reale; L Luciano
Journal: Histochemie Date: 1967

4. Synthesis of virus-specific proteins in Escherichia coli infected with the RNA bacteriophage MS2.

Authors: E Viñuela; I D Algranati; S Ochoa
Journal: Eur J Biochem Date: 1967-03

5. Human alkaline phosphatase. Immunochemical identification of organ-specific isoenzymes.

Authors: H H Sussman; P A Small; E Cotlove
Journal: J Biol Chem Date: 1968-01-10 Impact factor: 5.157

6. An estimate of the catalytic-centre activity and purity of human alkaline phosphatase preparations.

Authors: D W Moss; R H Eaton; P B Scutt
Journal: Biochim Biophys Acta Date: 1968-04-09

7. Studies on human placental alkaline phosphatase. I. Purification and crystallization.

Authors: E R Harkness
Journal: Arch Biochem Biophys Date: 1968-08 Impact factor: 4.013

8. Human kidney and urinary alkaline phosphatases.

Authors: P J Butterworth
Journal: Biochem J Date: 1968-04 Impact factor: 3.857

9. Viscosity and density of aqueous solutions of urea and guanidine hydrochloride.

Authors: K Kawahara; C Tanford
Journal: J Biol Chem Date: 1966-07-10 Impact factor: 5.157

10. An aminopeptidase occurring in pig kidney. I. An improved method of preparation. Physical and enzymic properties.

Authors: E D Wachsmuth; I Fritze; G Pfleiderer
Journal: Biochemistry Date: 1966-01 Impact factor: 3.162

11 in total

1. Evidence for the importance of arginine residues in pig kidney alkaline phosphatase.

Authors: M N Woodroofe; P J Butterworth
Journal: Biochem J Date: 1979-07-01 Impact factor: 3.857

2. Differentiation of epithelial cells in human jejunum: localization and quantification of aminopeptidase, alkaline phosphatase and aldolase isozymes in tissue sections.

Authors: E D Wachsmuth
Journal: Histochemistry Date: 1976-08-12

7. Human liver alkaline phosphatase purified by affinity chromatography, ultracentrifugation and polyacrylamide-gel electrophoresis.

Authors: A L Latner; A W Hodson
Journal: Biochem J Date: 1976-12-01 Impact factor: 3.857

Alkaline phosphatase from pig kidney. Method of purification and molecular properties.

1. The purification of aklaline phosphatases of animal tissues.

2. [Morphological and biochemical studies on the surface structure of the rat kidney brush-border membrane].

3. [Critical electron microscopical studies on the localization of activity of alkaline phosphatase in the main portion of the kidney of mice].

4. Synthesis of virus-specific proteins in Escherichia coli infected with the RNA bacteriophage MS2.

5. Human alkaline phosphatase. Immunochemical identification of organ-specific isoenzymes.

6. An estimate of the catalytic-centre activity and purity of human alkaline phosphatase preparations.

7. Studies on human placental alkaline phosphatase. I. Purification and crystallization.

8. Human kidney and urinary alkaline phosphatases.

9. Viscosity and density of aqueous solutions of urea and guanidine hydrochloride.

10. An aminopeptidase occurring in pig kidney. I. An improved method of preparation. Physical and enzymic properties.

1. Evidence for the importance of arginine residues in pig kidney alkaline phosphatase.

2. Differentiation of epithelial cells in human jejunum: localization and quantification of aminopeptidase, alkaline phosphatase and aldolase isozymes in tissue sections.

3. The mechanism of hydrolysis of beta-glycerophosphate by kidney alkaline phosphatase.

4. Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C.

5. Alkaline phosphates from human milk. Comparison with isoenzymes from placenta and liver.

6. Quantification of nephrotoxicity in rabbits by automated morphometry of alkaline phosphatase stained kidney sections.

7. Human liver alkaline phosphatase purified by affinity chromatography, ultracentrifugation and polyacrylamide-gel electrophoresis.

8. Alkaline phosphatase from pig kidney. Microheterogeneity and the role of neuraminic acid.

9. Characterization of phosphate binding by alkaline phosphatase in rat kidney brush border membrane.

10. Catalytic properties of alkaline phosphatase from pig kidney.