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Literature DB >> 4453522

Evidence for two waves of induction of DNA enzymes in stimulated human lymphocytes.

G C Noy, L Dalpra, A M Pedrini, G Ciarrocchi, E Giulotto, F Nuzzo, A Falaschi.

Abstract

The stimulation of human lymphocytes with phytohaemoagglutinin induces the appearance or increase of several enzymes of DNA metabolism [Pedrini etal., Biochem. Biophys. Res. Comm., 47:1221(1972)]. With long times of stimulation, two phenomena are observed; an increase in the levels of DNA polymerase, of a DNase acting on single-stranded DNA, and of an endonuclease, occurring between the third and fourth day, in parallel with a wave of DNA synthesis;a second wave of increase of the same enzymes and of DNA ligase,occurring between the fifth and eight day when the DNA replication rate, as measured by thymidine-pulses, has decreased to values close to the background.

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Year: 1974 PMID： 4453522 PMCID： PMC344339 DOI： 10.1093/nar/1.9.1183

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

Evidence for two waves of induction of DNA enzymes in stimulated human lymphocytes.

1. Induction of DNA polymerase in mouse L cells.

2. DNA polymerase activity in phytohemagglutinin-stimulated and non-stimulated human lymphocytes.

3. Purification and properties of a polynucleotide ligase from human cell cultures.

4. Induction of polynucleotide ligase in human lymphocytes stimulated by phytohemoagglutinin.

5. DNA polymerase activity of cultured normal and leukemic lymphocytes. Response to phytohemagglutinin.

6. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.

7. Properties of deoxyribonuclease 3 from mammalian tissues.

8. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

9. Induction of DNA polymerase in human lymphocytes by phytohemagglutinin.

10. Two forms of the DNA ligase of human cells.

1. Repair of ionizing radiation induced DNA damage in human lymphocytes.

2. A simplified procedure for the analysis of DNA polymerase III levels in Bacillus subtilis strains.

3. DNA ligase I gene expression during differentiation and cell proliferation.

4. Cell-cycle dependence and properties of the HeLa cell DNA polymerase system.

5. Variations of DNA polymerase-alpha and -beta during prolonged stimulation of human lymphocytes.