A sensitive method for the assay of oxytocin in the circulating blood.

1 The sensitivity of a wide range of isolated tissues to oxytocin was investigated. The longitudinal muscle strip of duck pulmonary vein proved the most suitable tissue for use with the superfusion technique, contracting to concentrations to oxytocin as low as 10 muu/ml.2 The duck pulmonary vein superfused with Krebs solution was contracted by oxytocin, the vasopressins, adrenaline, noradrenaline, 5-hydroxytryptamine, histamine and angiotensin II. Pre-treatment of the preparation with phenoxybenzamine (1-2 mug/ml) abolished the contractions to catecholamines and reduced the effects of histamine and 5-hydroxytryptamine without affecting the sensitivity to oxytocin.3 The pulmonary vein contracted when superfused with blood from an anaesthetized dog. This contraction was accompanied by a non-specific loss of responsiveness. When the pulmonary vein was superfused with Krebs solution that had been dialysed against blood the initial contraction was greatly reduced or abolished as was the loss in responsiveness.4 Oxytocin was stable in circulating dog's blood but approximately 50% was bound to plasma proteins. Oxytocin was not destroyed in the pulmonary circulation or the hind limbs. In the doses used oxytocin had a half-life of 60-90 s in the circulation of the dog under steady-state conditions. Disappearance occurred chiefly in the visceral vascular beds.