Literature DB >> 4451549

An improved micro-method for avidin assay.

H Elo, P Tuohimaa.   

Abstract

A sensitive method for avidin assay was devised. Tritiated biotin is bound to avidin and this complex is then bound to bentonite. Radioactivity is converted into a gas form by combustion and counted in an automatic proportional counter with 55% efficiency and background of 3.7c.p.m. The sensitivity is 1-2ng of avidin.

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Year:  1974        PMID： 4451549      PMCID： PMC1167978          DOI： 10.1042/bj1400115

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  3 in total

1.  The avidin-biotin equilibrium.

Authors:  H F LAUNER; H FRAENKEL-CONRAT
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Steroid hormone induction of a specific translatable messenger RNA.

Authors:  B W O'Malley; G C Rosenfeld; J P Comstock; A R Means
Journal:  Nat New Biol       Date:  1972-11-08

3.  An automatic gas counter for quantitative micro-determinations of tritium in biological material.

Authors:  M Parvinen; E Soini; P Tuohimaa
Journal:  Anal Biochem       Date:  1973-09       Impact factor: 3.365
  3 in total
  1 in total

1.  A radioimmunoassay for chicken avidin. Comparison with a [14C]biotin-binding method.

Authors:  M S Kulomaa; H A Elo; P J Tuohimaa
Journal:  Biochem J       Date:  1978-11-01       Impact factor: 3.857
  1 in total

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