Literature DB >> 444210

Comparison of endogenous and exogenous RNA primers of poly(U) polymerase in rat hepatic ribosomes.

T T Hayashi, K MacFarlane.   

Abstract

The present work indicates that RNA primer requirements for poly(U) polymerase in the free ribosomes of the rat liver depend upon the degree of enzyme purification. The poly(U) polymerase activity obtained from a crude free ribosomal preparation was compared with the enzymic activity of a partially purified enzyme. After preliminary purification, the enzyme was fractionated by chromatography on Sephadex G-150 and CM-cellulose. Our results demonstrate the presence of several forms of poly(U) polymerase activities, some requiring exogenous RNA and others possessing their own endogenous primer RNA.

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Year:  1979        PMID： 444210      PMCID： PMC1186455          DOI： 10.1042/bj1770895

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

1.  The uptake of ribonucleotide 5'-trephosphates by nuclear ribosomes.

Authors:  R H BURDON
Journal:  Biochem Biophys Res Commun       Date:  1963-06-20       Impact factor: 3.575

2.  The incorporation of uridine 5'-triphosphate into ribonucleic acid by enzyme fractions from Ehrlich ascites carcinoma cells.

Authors:  R H BURDON; R M SMELLIE
Journal:  Biochim Biophys Acta       Date:  1961-07-22

3.  ISOLATION CHARACTERISTICS OF RAPIDLY LABELLED RNA FROM NORMAL RAT LIVER.

Authors:  C KIDSON; K S KIRBY; R K RALPH
Journal:  J Mol Biol       Date:  1963-09       Impact factor: 5.469

4.  The incorporation of uridine 5'-phosphate into ribonucleic acid by an enzyme preparation from rat liver.

Authors:  H G KLEMPERER
Journal:  Biochim Biophys Acta       Date:  1963-07-30

5.  The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.

Authors:  A FLECK; H N MUNRO
Journal:  Biochim Biophys Acta       Date:  1962-05-14

6.  Polynucleotide biosynthesis: formation of a sequence of adenylate units from adenosine triphosphate by an enzyme from thymus nuclei.

Authors:  M EDMONDS; R ABRAMS
Journal:  J Biol Chem       Date:  1960-04       Impact factor: 5.157

7.  Nucleotide metabolism. II. Chromatographic separation of acid-soluble nucleotides.

Authors:  R B HURLBERT; H SCHMITZ; A F BRUMM; V R POTTER
Journal:  J Biol Chem       Date:  1954-07       Impact factor: 5.157

8.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

9.  Association of poly(A) and poly(U) polymerases with cytoplasmic ribosomes.

Authors:  G I Milchev; A A Hadjiolov
Journal:  Eur J Biochem       Date:  1978-03

10.  Poly(uridylic acid) sequences in messenger ribonucleic acid of HeLa cells.

Authors:  E L Korwek; N Nakazato; M Edmonds; S Venkatesan
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162
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  5 in total

1.  RNA end-labeling and RNA ligase activities can produce a circular rRNA in whole cell extracts from trypanosomes.

Authors:  T C White; P Borst
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971

2.  Control of activation of liver RNA polymerase I occurring after re-feeding of protein-depleted mice.

Authors:  L Haim; S Iapalucci-Espinoza; R Conde; M T Franze-Fernández
Journal:  Biochem J       Date:  1983-03-15       Impact factor: 3.857

3.  Purification of a terminal uridylyltransferase that acts as host factor in the in vitro poliovirus replicase reaction.

Authors:  N C Andrews; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

4.  Synthesis of virus-specific RNA in permeabilized murine coronavirus-infected cells.

Authors:  J L Leibowitz; J R DeVries
Journal:  Virology       Date:  1988-09       Impact factor: 3.616

5.  Gradual processing of the ITS1 from the nucleolus to the cytoplasm during synthesis of the human 18S rRNA.

Authors:  Milena Preti; Marie-Françoise O'Donohue; Nathalie Montel-Lehry; Marie-Line Bortolin-Cavaillé; Valérie Choesmel; Pierre-Emmanuel Gleizes
Journal:  Nucleic Acids Res       Date:  2013-03-12       Impact factor: 16.971
  5 in total

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