Literature DB >> 4434

Relationship between hemagglutinin and sialidase from Clostridium perfringens CN3870: chromatographic characterization of the biologically active proteins.

J I Rood, R G Wilkinson.   

Abstract

Biochemical characterization of hemagglutinin and sialidase activities from Clostridium perfringens strain CN3870 revealed that this strain produced three sialidase enzymes that were separable to gel filtration, ion exchange chromatography, and polyacrylamide gel electrophoresis. The molecular weights of sialidase I, II, and III activities were 310,000 +/- 10,000, 105,000 +/- 4,000 and 64,000 +/- 2,000, respectively, the first figure being an approximate value only.

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Year:  1976        PMID： 4434      PMCID： PMC233220          DOI： 10.1128/jb.126.2.831-844.1976

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  20 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Isolation and characterization of Clostridium perfringens mutants altered in both hemagglutinin and sialidase production.

Authors:  J I Rood; R G Wilkinson
Journal:  J Bacteriol       Date:  1975-08       Impact factor: 3.490

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Inhibition of neuraminidase activity by derivatives of 2-deoxy-2,3-dehydro-N-acetylneuraminic acid.

Authors:  P Meindl; G Bodo; P Palese; J Schulman; H Tuppy
Journal:  Virology       Date:  1974-04       Impact factor: 3.616

5.  A simple method for the purification of commercial neuraminidase preparations free from proteases.

Authors:  M W Hatton; E Regoeczi
Journal:  Biochim Biophys Acta       Date:  1973-11-15

6.  Structural components of Sendai virus. Serological and physicochemical characterization of hemagglutinin subunit associated with neuraminidase activity.

Authors:  H Tozawa; M Watanabe; N Ishida
Journal:  Virology       Date:  1973-09       Impact factor: 3.616

7.  The hemagglutinating and neuraminidase protein of a paramyxovirus: interaction with neuraminic acid in affinity chromatography.

Authors:  A Scheid; P W Choppin
Journal:  Virology       Date:  1974-11       Impact factor: 3.616

8.  Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein.

Authors:  A Scheid; L A Caliguiri; R W Compans; P W Choppin
Journal:  Virology       Date:  1972-12       Impact factor: 3.616

9.  The relationship of the haemagglutinin of Clostridium welchii to the neuraminidase and other soluble products of the organism.

Authors:  J G Collee
Journal:  J Pathol Bacteriol       Date:  1965-07

10.  [The estimation of the molecular weight of bacterial neuraminidases by gel-filtration (author's transl)].

Authors:  E Balke; W Scharmann; R Drzeniek
Journal:  Zentralbl Bakteriol Orig A       Date:  1974
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  4 in total

Review 1.  Toxigenic clostridia.

Authors:  C L Hatheway
Journal:  Clin Microbiol Rev       Date:  1990-01       Impact factor: 26.132

2.  Relationship between hemagglutinin and sialidase from Clostridium perfringens CN3870: gel filtration of mutant and reverant activities.

Authors:  J I Rood; R G Wilkinson
Journal:  J Bacteriol       Date:  1976-05       Impact factor: 3.490

3.  N-acetylglucosamine recognition by a family 32 carbohydrate-binding module from Clostridium perfringens NagH.

Authors:  Elizabeth Ficko-Blean; Alisdair B Boraston
Journal:  J Mol Biol       Date:  2009-05-05       Impact factor: 5.469

Review 4.  Molecular genetics and pathogenesis of Clostridium perfringens.

Authors:  J I Rood; S T Cole
Journal:  Microbiol Rev       Date:  1991-12
  4 in total

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