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Literature DB >> 4426983

Separation of abnormal cells in the peripheral blood by means of the zonal centrifuge.

Abstract

The basic principles of rate centrifugation in the large volume zonal centrifuge and its application to the separation of abnormal or rarely occurring cells in the blood are described. Details of the method, gradient media, and collection and examination of the fractions obtained are given. A description of the cells found in normal human blood, in a subject undergoing known immunological stimulus, and in glandular fever and Hodgkin's disease is given and the implications are discussed.

Entities: Disease Species

Mesh：

Substances：
Antigens
Smallpox Vaccine

Year: 1974 PMID： 4426983 PMCID： PMC475461 DOI： 10.1136/jcp.27.9.741

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

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References

17 in total

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Authors: T G Pretlow; C W Boone
Journal: Exp Mol Pathol Date: 1969-10 Impact factor: 3.362

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Authors: K Shortman
Journal: Aust J Exp Biol Med Sci Date: 1968-08

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Authors: R Harris; E O Ukaejiofo
Journal: Br J Haematol Date: 1970-02 Impact factor: 6.998

Review 4. Zonal centrifuges and other separation systems.

Authors: N G Anderson
Journal: Science Date: 1966-10-07 Impact factor: 47.728

5. Significance of the changes in the circulating lymphoid cells in Hodgkin's disease.

Authors: D Cowther; G H Fairley; R L Sewell
Journal: Br Med J Date: 1969-05-24

6. The selective elimination of immunologically competent cells from bone marrow and lymphocyte cell mixtures. 3. In vitro test for detection of immunocompetent cells in fractionated mouse spleen cell suspensions and primate bone marrow suspensions.

Authors: K A Dicke; G Tridente; D W van Bekkum
Journal: Transplantation Date: 1969-10 Impact factor: 4.939

Separation of abnormal cells in the peripheral blood by means of the zonal centrifuge.

1. Separation of mammalian cells using programmed gradient sedimentation.

2. The separation of different cell classes from lymphoid organs. II. The purification and analysis of lymphocyte populations by equilibrium density gradient centrifugation.

3. Tissue typing using a routine one-step lymphocyte separation procedure.

Review 4. Zonal centrifuges and other separation systems.

5. Significance of the changes in the circulating lymphoid cells in Hodgkin's disease.

6. The selective elimination of immunologically competent cells from bone marrow and lymphocyte cell mixtures. 3. In vitro test for detection of immunocompetent cells in fractionated mouse spleen cell suspensions and primate bone marrow suspensions.

7. Response of human blood lymphocytes to tuberculin PPD in tissue culture.

8. Sternberg-Reed cells in the peripheral blood of patients with Hodgkin's disease.

9. Characterization of macromolecules by constant velocity sedimentation.

10. The resolution of mixtures of viable mammalian cells into homogeneous fractions by zonal centrifugation.