| Literature DB >> 4420960 |
Abstract
A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but not by saponin, ribonuclease, phospholipase C, or high-frequency sonic treatment. Sonic treatment further fractionates the cell ghost and allows partial purification, on sucrose density gradients, of a deoxyribonucleic acid replication complex attached to the cells' polar membrane-bleb-infrableb structures.Entities:
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Year: 1974 PMID： 4420960 PMCID： PMC245788 DOI： 10.1128/jb.120.1.495-501.1974
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490