Literature DB >> 4394948

Purification and properties of the nicotinamide-adenine dinucleotide phosphate-dependent isocitrate dehydrogenase from pig liver cytoplasm.

J A Illingworth, K F Tipton.   

Abstract

The NADP-dependent isocitrate dehydrogenase from pig liver soluble fraction was purified over 500-fold with an overall yield of 25%. The purified enzyme, which is homogeneous by all the usual criteria, has a molecular weight of about 75000 and is composed of two identical subunits. This has been demonstrated by ultracentrifugation, fluorescence titration and peptide ;fingerprinting'. The maximal turnover number, extinction coefficients at 280nm and 260nm and amino acid analysis are described.

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Year:  1970        PMID： 4394948      PMCID： PMC1179111          DOI： 10.1042/bj1180253

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  The preparation of isocitric dehydrogenase from mammalian heart.

Authors:  G SIEBERT; J DUBUC; R C WARNER; G W PLAUT
Journal:  J Biol Chem       Date:  1957-06       Impact factor: 5.157

2.  Binding of substrates by native and chemically modified isocitrate dehydrogenase.

Authors:  R F Colman
Journal:  Biochim Biophys Acta       Date:  1969-11-04

3.  The subunits of porcine heart TPN-linked isocitrate dehydrogenase.

Authors:  M E Magar; J E Robbins
Journal:  Biochim Biophys Acta       Date:  1969-09-30

4.  Allosteric inhibition of TPN-linked isocitrate dehydrogenase by folate.

Authors:  M E Magar; M L Homi
Journal:  Biochem Biophys Res Commun       Date:  1968-06-10       Impact factor: 3.575

5.  Deuterium solvent isotope effects in reactions catalyzed.

Authors:  R F Colman; R Chu
Journal:  Biochem Biophys Res Commun       Date:  1969-02-21       Impact factor: 3.575

6.  Effect of modification of a methionyl residue on the kinetic and molecular properties of isocitrate dehydrogenase.

Authors:  R F Colman
Journal:  J Biol Chem       Date:  1968-05-25       Impact factor: 5.157

7.  Evaluation of diffusion coefficients of proteins from sedimentation boundary curves.

Authors:  K Kawahara
Journal:  Biochemistry       Date:  1969-06       Impact factor: 3.162

8.  Evidence for a methionyl residue in the active site of isocitrate dehydrogenase.

Authors:  R F Colman
Journal:  Biochem Biophys Res Commun       Date:  1967-07-21       Impact factor: 3.575

9.  The role of sulfhydryl groups in the catalytic function of isocitrate dehydrogenase. I. Reaction with 5,5'-dithiobis(2-nitrobenzoic acid).

Authors:  R F Colman
Journal:  Biochemistry       Date:  1969-03       Impact factor: 3.162

10.  Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors:  P Andrews
Journal:  Biochem J       Date:  1964-05       Impact factor: 3.766
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  10 in total

1.  Comparative biochemical studies of isozymes of isocitrate dehydrogenase from the mouse.

Authors:  B Pegoraro; C Y Lee
Journal:  Mol Cell Biochem       Date:  1979-03-05       Impact factor: 3.396

2.  Molecular weight of NADP-dependent isocitrate dehydrogenase from rat brain cytosol under normoxia and hypoxia.

Authors:  U Rafałowska; A Pastuszko
Journal:  Neurochem Res       Date:  1979-04       Impact factor: 3.996

3.  Bistability in the isocitrate dehydrogenase reaction: an experimentally based theoretical study.

Authors:  G M Guidi; M F Carlier; A Goldbeter
Journal:  Biophys J       Date:  1998-03       Impact factor: 4.033

4.  Purification and structural properties of isozymes of isocitrate dehydrogenase from the mouse.

Authors:  B Pegoraro; J H Yuan; C Y Lee
Journal:  Mol Cell Biochem       Date:  1979-02-09       Impact factor: 3.396

5.  Chromatographic and immunological evidence that chloroplastic and cytosolic pea (Pisum sativum L.) NADP-isocitrate dehydrogenases are distinct isoenzymes.

Authors:  R D Chen; E Bismuth; M L Champigny; P Gadal
Journal:  Planta       Date:  1989-05       Impact factor: 4.116

6.  Kinetics and Thermostability of NADP-Isocitrate Dehydrogenase from Cephalosporium acremonium.

Authors:  J Olano; D de Arriaga; F Busto; J Soler
Journal:  Appl Environ Microbiol       Date:  1995-06       Impact factor: 4.792

7.  Novel NADP-linked isocitrate dehydrogenase present in peroxisomes of n-alkane-utilizing yeast, Candida tropicalis: comparison with mitochondrial NAD-linked isocitrate dehydrogenase.

Authors:  S Yamamoto; H Atomi; M Ueda; A Tanaka
Journal:  Arch Microbiol       Date:  1995-02       Impact factor: 2.552

8.  Purification of yeast isocitrate dehydrogenase.

Authors:  J A Illingworth
Journal:  Biochem J       Date:  1972-10       Impact factor: 3.857

9.  Purification and comparative properties of isoenzymes of nicotinamide-adenine dinucleotide phosphate-isocitrate dehydrogenase from rat heart and liver.

Authors:  M Islam; J L Bell; D N Baron
Journal:  Biochem J       Date:  1972-10       Impact factor: 3.857

10.  A pyrosequencing-based assay for the rapid detection of IDH1 mutations in clinical samples.

Authors:  Prashanth Setty; Jennifer Hammes; Thomas Rothämel; Valentina Vladimirova; Christof M Kramm; Torsten Pietsch; Andreas Waha
Journal:  J Mol Diagn       Date:  2010-09-16       Impact factor: 5.341
  10 in total

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