Literature DB >> 4372626

Establishment of a clone of mouse hypothalamic neurosecretory cells synthesizing neurophysin and vasopressin.

F De Vitry, M Camier, P Czernichow, P Benda, P Cohen, A Tixier-Vidal.   

Abstract

Hypothalamic cells taken from 14-day-old mouse embryos were cultured for 6 days and transformed with simian virus 40. After cloning, a homogeneous cell population was obtained. Its morphological, Ultrastructural, biochemical, and immunochemical properties were studied. These cells possess ultrastructural features of primitive neurosecretory cells. They synthesize (35)S-labeled protein components that have the molecular weight and isoelectric focusing behavior of, and display the same immunoreactivity as, neurophysin. In addition, a (35)S-labeled peptidic fraction with a molecular weight close to 1000 is synthesized and is radioimmunologically indistinguishable from vasopressin. Immunochemical staining shows that both neurophysin and vasopressin are localized in the cytoplasm. These observations strongly suggest that a clone of mouse hypothalamic neurosecretory cells has been obtained with the synthesizing capacities of secretory neurons of the magnocellular hypothalamic nuclei.

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Year:  1974        PMID： 4372626      PMCID： PMC433817          DOI： 10.1073/pnas.71.9.3575

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  7 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Hormonal interactions at the molecular level. A study of oxytocin and vasopressin binding to bovine neurophysins.

Authors:  M Camier; R Alazard; P Cohen
Journal:  Eur J Biochem       Date:  1973-01-15

3.  The use of thyroglobulin to induce antigenicity to small molecules.

Authors:  W R Skowsky; D A Fisher
Journal:  J Lab Clin Med       Date:  1972-07

4.  A correlation of release of polypeptide hormones and of immunoreactive neurophysin from isolated rat neurohypophyses.

Authors:  J J Nordmann; J J Dreifuss; J J Legros
Journal:  Experientia       Date:  1971

5.  Immunochemical analysis of rabbit antibodies against vasopressin.

Authors:  P Czernichow; A Reinharz; M B Vallotton
Journal:  Immunochemistry       Date:  1974-01

6.  The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Crystallization of neurophysin-I and-II as complexes with [8-arginine]-vasopressin.

Authors:  M D Hollenberg; D B Hope
Journal:  Biochem J       Date:  1968-01       Impact factor: 3.857

7.  Procedure for embedding in situ selected cells cultured in vitro.

Authors:  B R Brinkley; P Murphy; L C Richardson
Journal:  J Cell Biol       Date:  1967-10       Impact factor: 10.539
  7 in total
  9 in total

1.  Dissociated cell cultures from fetal mouse hypothalamus. Patterns of organization and ultrastructural features.

Authors:  P Benda; F De Vitry; R Picart; A Tixier-Vidal
Journal:  Exp Brain Res       Date:  1975-07-11       Impact factor: 1.972

2.  Ultrastructural and cytochemical features of SV 40 transformed hypothalamic cell lines.

Authors:  A Tixier-Vidal; F De Vitry
Journal:  Cell Tissue Res       Date:  1976-08-16       Impact factor: 5.249

Review 3.  Physiological relevance and functional potential of central nervous system-derived cell lines.

Authors:  S R Whittemore; E Y Snyder
Journal:  Mol Neurobiol       Date:  1996-02       Impact factor: 5.590

Review 4.  Human cell transformation by simian virus 40--a review.

Authors:  G H Sack
Journal:  In Vitro       Date:  1981-01

5.  Establishment of rat pancreatic endocrine cell lines by infection with simian virus 40.

Authors:  E J Niesor; C B Wollheim; D H Mintz; B Blondel; A E Renold; R Weil
Journal:  Biochem J       Date:  1979-03-15       Impact factor: 3.857

6.  Hormonal regulation of clonal, immortalized hypothalamic neurons expressing neuropeptides involved in reproduction and feeding.

Authors:  Denise D Belsham
Journal:  Mol Neurobiol       Date:  2007-04       Impact factor: 5.590

7.  Serotonin initiates and autoamplifies its own synthesis during mouse central nervous system development.

Authors:  F De Vitry; M Hamon; J Catelon; M Dubois; J Thibault
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

8.  Establishment of 'normal' nervous cell lines after transfer of polyoma virus and adenovirus early genes into murine brain cells.

Authors:  C Evrard; E Galiana; P Rouget
Journal:  EMBO J       Date:  1986-12-01       Impact factor: 11.598

9.  Induction of oligodendrocyte-like properties in a primitive hypothalamic cell line by cholesterol, an eye derived growth factor and brain extract.

Authors:  F de Vitry; J P Delaunoy; J Thibault; N Buisson; N Lamande; L Legault; A Delasalle; P Dupouey
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
  9 in total

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