Literature DB >> 4368772

Histidine residues and the enzyme activity of pig heart supernatant malate dehydrogenase.

J J Holbrook, A Lodola, N P Illsley.   

Abstract

1. Supernatant pig heart malate dehydrogenase is completely inhibited by reaction with diethyl pyrocarbonate at pH6.5, when 0.58+/-0.1 residue of ethoxycarbonylhistidine is formed per NADH-binding site. 2. Oxaloacetate and hydroxymalonate protect the enzyme from inhibition in the absence of coenzyme. 3. Limited ethoxycarbonylation does not alter the binding of NADH to the enzyme but prevents the enzyme-NADH complex from interacting with hydroxymalonate in a ternary complex.

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Year:  1974        PMID： 4368772      PMCID： PMC1166348          DOI： 10.1042/bj1390797

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Approaches to the study of enzyme mechanisms lactate dehydrogenase.

Authors:  J J. Holbrook; H Gutfreund
Journal:  FEBS Lett       Date:  1973-04-15       Impact factor: 4.124

2.  Protein fluorescence of lactate dehydrogenase.

Authors:  J J Holbrook
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

3.  Polypeptide conformation of cytoplasmic malate dehydrogenase from an electron density map at 3.0 angstrom resolution.

Authors:  E Hill; D Tsernoglou; L Webb; L J Banaszak
Journal:  J Mol Biol       Date:  1972-12-30       Impact factor: 5.469

4.  Alkylation studies on a reactive histidine in pig heart malate dehydrogenase.

Authors:  B H Anderton; B R Rabin
Journal:  Eur J Biochem       Date:  1970-09

5.  Ethoxyformylation of proteins. Reaction of ethoxyformic anhydride with alpha-chymotrypsin, pepsin, and pancreatic ribonuclease at pH 4.

Authors:  W B Melchior; D Fahrney
Journal:  Biochemistry       Date:  1970-01-20       Impact factor: 3.162

6.  Malic dehydrogenase. 8. Large scale purification and properties of supernatant pig heart enzyme.

Authors:  R K Gerding; R G Wolfe
Journal:  J Biol Chem       Date:  1969-03-10       Impact factor: 5.157

7.  Structural studies of pig heart malate dehydrogenase.

Authors:  T Dévényi; S J Rogers; R G Wolfe
Journal:  Nature       Date:  1966-04-30       Impact factor: 49.962

8.  The reaction of a histidine residue in glutamate dehydrogenase with diethyl pyrocarbonate.

Authors:  R B Wallis; J J Holbrook
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

9.  Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase.

Authors:  J J Holbrook; V A Ingram
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857

10.  Malate dehydrogenase. X. Fluorescence microtitration studies of D-malate, hydroxymalonate, nicotinamide dinucleotide, and dihydronicotinamide-adenine dinucleotide binding by mitochondrial and supernatant porcine heart enzymes.

Authors:  J J Holbrook; R G Wolfe
Journal:  Biochemistry       Date:  1972-06-20       Impact factor: 3.162
  10 in total
  3 in total

1.  Reversible modification of pig heart mitochondrial malate dehydrogenase by pyridoxal 5'-phosphate.

Authors:  S S Chen; P C Engel
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

2.  Substrate-specificity of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by TOL plasmid pWW0. Metabolic and mechanistic implications.

Authors:  J P Shaw; F Schwager; S Harayama
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

3.  Malate dehydrogenase of the cytosol. Ionizations of the enzyme-reduced-coenzyme complex and a comparison with lactate dehydrogenase.

Authors:  A Lodola; D M Parker; R Jeck; J J Holbrook
Journal:  Biochem J       Date:  1978-08-01       Impact factor: 3.857
  3 in total

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