Literature DB >> 4368324

A demonstration of several deoxyribonuclease activities in mammalian cell mitochondria.

M Durphy, P N Manley, E C Friedberg.   

Abstract

Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts. Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria.

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Year:  1974        PMID： 4368324      PMCID： PMC2109224          DOI： 10.1083/jcb.62.3.695

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  20 in total

1.  Studies on the localization of rat liver mitochondrial 5'-endonuclease.

Authors:  R Morais
Journal:  Biochim Biophys Acta       Date:  1969-09-16

2.  Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes.

Authors:  S D Barbour; A J Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1970-04       Impact factor: 11.205

3.  An endonuclease activity from Escherichia coli absent from certain rec- strains.

Authors:  P J Goldmark; S Linn
Journal:  Proc Natl Acad Sci U S A       Date:  1970-09       Impact factor: 11.205

4.  Partial purification and some properties of a 5'-RNase from rat liver mitochondrial fraction.

Authors:  R Morais; M Blackstein; G de Lamirande
Journal:  Arch Biochem Biophys       Date:  1967-09       Impact factor: 4.013

5.  Complex mitochondrial DNA.

Authors:  B Hudson; D A Clayton; J Vinograd
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1968

6.  Occurrence of complex mitochondrial DNA in normal tissues.

Authors:  D A Clayton; C A Smith; J M Jordan; M Teplitz; J Vinograd
Journal:  Nature       Date:  1968-12-07       Impact factor: 49.962

7.  DNA polymerase activity in methotrexate plus uridine treated Tetrahymena.

Authors:  O Westergaard; R E Pearlman
Journal:  Exp Cell Res       Date:  1969-03       Impact factor: 3.905

8.  Differential release of alkaline deoxyribonuclease and of glutamate dehydrogenase from rat-liver mitochondrial fractions.

Authors:  P Baudhuin; E Hertoghe-Lefèvre; C De Duve
Journal:  Biochem Biophys Res Commun       Date:  1969-05-22       Impact factor: 3.575

9.  The purification from rat liver of a nuclease hydrolysing ribonucleic acid and deoxyribonucleic acid.

Authors:  P J Curtis; M G Burdon; R M Smellie
Journal:  Biochem J       Date:  1966-03       Impact factor: 3.857

10.  Complex mitochondrial DNA in leukemic and normal human myeloid cells.

Authors:  D A Clayton; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205
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  5 in total

Review 1.  Integration and regulation of mitochondrial assembly in yeast.

Authors:  H R Mahler; S H Phan; R N Bastos
Journal:  Mol Cell Biochem       Date:  1977-02-04       Impact factor: 3.396

2.  Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria.

Authors:  A E Tomkinson; S Linn
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

3.  Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae.

Authors:  R D Vincent; T J Hofmann; H P Zassenhaus
Journal:  Nucleic Acids Res       Date:  1988-04-25       Impact factor: 16.971

4.  A mitochondrial nuclease is modified in Drosophila mutants (mus308) that are hypersensitive to DNA crosslinking agents.

Authors:  K Sakaguchi; P V Harris; R van Kuyk; A Singson; J B Boyd
Journal:  Mol Gen Genet       Date:  1990-12

5.  The preference of the mitochondrial endonuclease for a conserved sequence block in mitochondrial DNA is highly conserved during mammalian evolution.

Authors:  R L Low; J M Buzan; C L Couper
Journal:  Nucleic Acids Res       Date:  1988-07-25       Impact factor: 16.971
  5 in total

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