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Literature DB >> 4362928

Two-hour embedding procedure for intracellular detection of viruses by electron microscopy.

F W Doane, N Anderson, J Chao, A Noonan.

Abstract

An embedding method requiring only 2 h to complete and giving excellent ultrastructural preservation has been used for the rapid detection of viruses in tissue cultures. The method has also been applied successfully to mammalian tissue. It provides a rapid technique for identifying viruses isolated in tissue cultures, for screening cultures for adventitious agents, and for examining tissue biopsies for viruses.

Entities: Species

Mesh：

Substances：
Oxides
Osmium
Glutaral

Year: 1974 PMID： 4362928 PMCID： PMC380043 DOI： 10.1128/am.27.2.407-410.1974

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

10 in total

1. Fast method for processing biologic material for electron microscopy.

Authors: S A Bencosme; V Tsutsumi
Journal: Lab Invest Date: 1970-10 Impact factor: 5.662

2. Rapid processing of kidney biopsies for electron microscopy.

Authors: J V Johannessen
Journal: Kidney Int Date: 1973-01 Impact factor: 10.612

3. Electron microscope methods for the identification of adenoviruses isolated in micro tissue cultures.

Authors: M J Rosenbaum; R C Kory; K A Siegesmund; H J Pedersen; E J Sullivan; R O Peckinpaugh
Journal: Appl Microbiol Date: 1972-01

4. Microscopic detection of adventitious viruses in cell cultures.

Authors: N Anderson; F W Doane
Journal: Can J Microbiol Date: 1972-03 Impact factor: 2.419

5. A rapid embedding technique for electron microscopy.

Authors: L W Estes; J V Apicella
Journal: Lab Invest Date: 1969-02 Impact factor: 5.662

6. A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors: A R Spurr
Journal: J Ultrastruct Res Date: 1969-01

7. Rapid and improved methods fr embedding biological tissues in Epon 812 and Araldite 502.

Authors: H D Coulter
Journal: J Ultrastruct Res Date: 1967-10-31

8. Application of electron microscopy to the diagnosis of virus infections.

Authors: F W Doane; N Anderson; A Zbitnew; A J Rhodes
Journal: Can Med Assoc J Date: 1969-06-14 Impact factor: 8.262

9. New ultrastructural characteristics of cells fixed in a glutaraldehyde-osmium tetroxide mixture.

Authors: B F Trump; R E Bulger
Journal: Lab Invest Date: 1966-01 Impact factor: 5.662

10. Agar diffusion method for negative staining of microbial suspensions in salt solutions.

Authors: N Anderson; F W Doane
Journal: Appl Microbiol Date: 1972-09

10 in total

12 in total

Two-hour embedding procedure for intracellular detection of viruses by electron microscopy.

1. Fast method for processing biologic material for electron microscopy.

2. Rapid processing of kidney biopsies for electron microscopy.

3. Electron microscope methods for the identification of adenoviruses isolated in micro tissue cultures.

4. Microscopic detection of adventitious viruses in cell cultures.

5. A rapid embedding technique for electron microscopy.

6. A low-viscosity epoxy resin embedding medium for electron microscopy.

7. Rapid and improved methods fr embedding biological tissues in Epon 812 and Araldite 502.

8. Application of electron microscopy to the diagnosis of virus infections.

9. New ultrastructural characteristics of cells fixed in a glutaraldehyde-osmium tetroxide mixture.

10. Agar diffusion method for negative staining of microbial suspensions in salt solutions.

1. Serospecificity and opsonic activity of antisera to Legionella pneumophila.

Review 2. Modern uses of electron microscopy for detection of viruses.

3. Electron microscopy of rapid identification of animal viruses in hematoxylin-eosin sections.

4. Application of electron microscopy in viral diagnosis.

5. Virus morphology as an aid for rapid diagnosis.

Review 6. Electron microscopy for the rapid detection and identification of viruses from clinical specimens.

7. Methods for rapid detection of human ocular viral infections.

8. "Runde" viurs, a coronavirus-like agent associated with seabirds and ticks.

9. Rapid diagnostic thin section electron microscopy of bacterial endospores.

10. Detection and identification of viruses by electron microscopy.