Literature DB >> 4362872

Immunochemical procedure for partial purification of newly synthesized proteins in polyoma virus-infected mouse cells.

D Paulin, J Perreau, F Cuzin.   

Abstract

A broad-spectrum antiserum has been obtained against nuclear proteins from normal mouse fibroblasts. Solid phase immunoadsorbants prepared from the serum specifically bind the corresponding antigens. The removal in this way of a significant fraction of host proteins leads to partial purification of viral and virus-induced polypeptides from polyoma virus-infected cell extracts. Four main peptides can be selected, with respective molecular weights of 90,000, 70,000, 46,000, and 41,000.

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Year:  1974        PMID: 4362872      PMCID: PMC355356     

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  17 in total

1.  Polyoma virus basic proteins.

Authors:  P M Frearson; L V Crawford
Journal:  J Gen Virol       Date:  1972-02       Impact factor: 3.891

2.  Immediate visualization of proteins in dodecyl sulfate-polyacrylamide gels by prestaining with Remazol dyes.

Authors:  I P Griffith
Journal:  Anal Biochem       Date:  1972-04       Impact factor: 3.365

3.  Polyoma virus proteins. 1. Multiple virion components.

Authors:  R Roblin; E Härle; R Dulbecco
Journal:  Virology       Date:  1971-09       Impact factor: 3.616

4.  The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.

Authors:  S Avrameas; T Ternynck
Journal:  Immunochemistry       Date:  1969-01

5.  Localization of virus antigens by enzyme-labelled antibodies.

Authors:  R Wicker; S Avrameas
Journal:  J Gen Virol       Date:  1969-06       Impact factor: 3.891

6.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157

7.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

8.  Identification of virus-induced proteins in cells productively infected with simian virus 40.

Authors:  H Fischer; G Sauer
Journal:  J Virol       Date:  1972-01       Impact factor: 5.103

9.  Protein synthesis in Simian virus 40-infected monkey cells.

Authors:  G Walter; R Roblin; R Dulbecco
Journal:  Proc Natl Acad Sci U S A       Date:  1972-04       Impact factor: 11.205

10.  Pattern of protein synthesis in monkey cells infected by simian virus 40.

Authors:  C W Anderson; R F Gesteland
Journal:  J Virol       Date:  1972-05       Impact factor: 5.103

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  2 in total

1.  Polyoma virus T antigen. I. Synthesis of modified heat-labile T angiten in cells transformed with the ts-a mutant.

Authors:  D Paulin; F Cuzin
Journal:  J Virol       Date:  1975-02       Impact factor: 5.103

2.  Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.

Authors:  B S Schaffhausen; J E Silver; T L Benjamin
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

  2 in total

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