Literature DB >> 4362496

A rapid method for collecting and assaying gradient fractions for radioactive macromolecules.

L Rubinstein, A Rein.   

Abstract

A rapid method is described for collecting gradients after centrifugation, and assaying them for acid-precipitable radioactivity. An entire gradient is collected and processed on a single glass-fibre filter disc. This method allows one easily to assay six gradients for acid-precipitable radioactivity in less than 2h.

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Year:  1974        PMID： 4362496      PMCID： PMC1166092          DOI： 10.1042/bj1370131

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  6 in total

1.  Band-centrifugation of macromolecules and viruses in self-generating density gradients.

Authors:  J VINOGRAD; R BRUNER; R KENT; J WEIGLE
Journal:  Proc Natl Acad Sci U S A       Date:  1963-06       Impact factor: 11.205

2.  Thermal conversion of nonpriming deoxyribonucleic acid to primer.

Authors:  F J BOLLUM
Journal:  J Biol Chem       Date:  1959-10       Impact factor: 5.157

3.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

4.  A very rapid method for washing large numbers of precipitates of proteins and nucleic acids.

Authors:  A V Furano
Journal:  Anal Biochem       Date:  1971-10       Impact factor: 3.365

5.  Paper strip method for assaying gradient fractions containing radioactive macromolecules.

Authors:  W L Carrier; R B Setlow
Journal:  Anal Biochem       Date:  1971-10       Impact factor: 3.365

6.  Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces.

Authors:  R A McGrath; R W Williams
Journal:  Nature       Date:  1966-10-29       Impact factor: 49.962
  6 in total

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