Literature DB >> 4356058

Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase.

J J Holbrook.   

Abstract

With the use of a glass electrode it is shown that one proton is taken up from the solvent when o-nitrophenylpyruvate forms a ternary complex with lactate dehydrogenase and NADH at pH8 and before the complex has rearranged to give products.

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Year:  1973        PMID： 4356058      PMCID： PMC1177776          DOI： 10.1042/bj1330847

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  7 in total

1.  Approaches to the study of enzyme mechanisms lactate dehydrogenase.

Authors:  J J. Holbrook; H Gutfreund
Journal:  FEBS Lett       Date:  1973-04-15       Impact factor: 4.124

2.  Structural constraints of possible mechanisms of lactate dehydrogenase as shown by high resolution studies of the apoenzyme and a variety of enzyme complexes.

Authors:  M G Rossmann; M J Adams; M Buehner; G C Ford; M L Hackert; P J Lentz; A McPherson; R W Schevitz; I E Smiley
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1972

3.  [Labelling of essential amino acid residues of pig heart lactate dehydrogenase with (carbonyl-14C)3-(2-bromoacetyl) pyridine].

Authors:  C Woenckhaus; J Berghäuser; G Pfleiderer
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1969-04

4.  Lactic dehydrogenase. X. A re-evaluation of the effects of pH upon the kinetics of the reaction.

Authors:  G W Schwert; B R Miller; R J Peanasky
Journal:  J Biol Chem       Date:  1967-07-25       Impact factor: 5.157

5.  Equilibrium binding of nicotinamide nucleotides to lactate dehydrogenases.

Authors:  R A Stinson; J J Holbrook
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857

6.  The use of ternary complexes to study ionizations and isomerizations during catalysis by lactate dehydrogenase.

Authors:  J J Holbrook; R A Stinson
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857

7.  Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase.

Authors:  J J Holbrook; V A Ingram
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857
  7 in total
  2 in total

1.  A detailed investigation of the properties of lactate dehydrogenase in which the 'Essential' cysteine-165 is modified by thioalkylation.

Authors:  D P Bloxham; R P Sharma; D C Wilton
Journal:  Biochem J       Date:  1979-03-01       Impact factor: 3.857

2.  Charge balance in the alpha-hydroxyacid dehydrogenase vacuole: an acid test.

Authors:  A Cortes; D C Emery; D J Halsall; R M Jackson; A R Clarke; J J Holbrook
Journal:  Protein Sci       Date:  1992-07       Impact factor: 6.725
  2 in total

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