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Literature DB >> 4351176

Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts.

L Jiménez de Asúa, E S Surian, M M Flawia, H N Torres.

Abstract

BHK 21/13 fibroblasts grown in the presence of insulin show some characteristics of a transformed strain. Cells broaden, cross each other intricately, and form colonies on both polystyrene dishes and soft agar. The effect of insulin is prevented by addition of N(6)-butyryl adenosine 3':5'-cyclic monophosphate and theophylline to the culture medium. Insulin causes a decrease in the concentration of intracellular adenosine 3':5'-cyclic monophosphate and inhibition of adenylate cyclase activity in BHK fibroblast membranes. This inhibition is observed at insulin concentrations of 0.1 nM or higher.

Entities: Chemical Gene

Mesh：

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Year: 1973 PMID： 4351176 PMCID： PMC433503 DOI： 10.1073/pnas.70.5.1388

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

22 in total

1. Activation of calcium efflux by ADP and inorganic phosphate.

Authors: B Barlogie; W Hasselbach; M Makinose
Journal: FEBS Lett Date: 1971-01-30 Impact factor: 4.124

2. Growth control and cyclic alterations of cyclic AMP in the cell cycle.

Authors: M M Burger; B M Bombik; B M Breckenridge; J R Sheppard
Journal: Nat New Biol Date: 1972-10-11

3. A mutation in a rous sarcoma virus gene that controls adenosine 3',5'-monophosphate levels and transformation.

Authors: J Otten; J Bader; G S Johnson; I Pastan
Journal: J Biol Chem Date: 1972-03-10 Impact factor: 5.157

4. Adenyl cyclase in normal and transformed fibroblasts in tissue culture. Activation by prostaglandins.

Authors: C V Peery; G S Johnson; I Pastan
Journal: J Biol Chem Date: 1971-09-25 Impact factor: 5.157

5. Studies on carcinogenesis by avian sarcoma viruses. VI. Differential multiplication of uninfected and of converted cells in response to insulin.

Authors: H M Temin
Journal: J Cell Physiol Date: 1967-06 Impact factor: 6.384

6. Role of adenosine 3',5'-monophosphate in the effects of insulin and anti-insulin serum on liver metabolism.

Authors: L S Jefferson; J H Exton; R W Butcher; E W Sutherland; C R Park
Journal: J Biol Chem Date: 1968-03-10 Impact factor: 5.157

7. Effect of insulin on adenosine 3',5'-monophosphate in the rat epididymal fat pad.

Authors: R W Butcher; J G Sneyd; C R Park; E W Sutherland
Journal: J Biol Chem Date: 1966-04-10 Impact factor: 5.157

8. The initiation of cell division in a contact-inhibited mammalian cell line.

Authors: G J Todaro; G K Lazar; H Green
Journal: J Cell Physiol Date: 1965-12 Impact factor: 6.384

9. Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3':5'-monophosphate and testosterone.

Authors: A W Hsie; T T Puck
Journal: Proc Natl Acad Sci U S A Date: 1971-02 Impact factor: 11.205

10. Role of cyclic 3':5'-adenosine monophosphate in the early transport changes induced by serum and insulin in quiescent fibroblasts.

Authors: E Rozengurt; L Jimenez de Asua
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts.

1. Activation of calcium efflux by ADP and inorganic phosphate.

2. Growth control and cyclic alterations of cyclic AMP in the cell cycle.

3. A mutation in a rous sarcoma virus gene that controls adenosine 3',5'-monophosphate levels and transformation.

4. Adenyl cyclase in normal and transformed fibroblasts in tissue culture. Activation by prostaglandins.

5. Studies on carcinogenesis by avian sarcoma viruses. VI. Differential multiplication of uninfected and of converted cells in response to insulin.

6. Role of adenosine 3',5'-monophosphate in the effects of insulin and anti-insulin serum on liver metabolism.

7. Effect of insulin on adenosine 3',5'-monophosphate in the rat epididymal fat pad.

8. The initiation of cell division in a contact-inhibited mammalian cell line.

9. Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3':5'-monophosphate and testosterone.

10. Restoration of contact-inhibited growth to transformed cells by dibutyryl adenosine 3':5'-cyclic monophosphate.

1. Cyclic AMP and cyclic GMP concentrations in serum- and density-restricted fibroblast cultures.

2. Inhibition of replication of normal adrenocortical cells in culture by adrenocorticotropin.

Review 3. Studies on the mechanism of insulin action: basic concepts and clinical implications.

4. Surface morphology of normal and neoplastic rat cells.

5. Intracellular cyclic AMP concentration responds specifically to growth regulation by serum.

6. Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin.

7. Growth of a rat neuroblastoma cell line in serum-free supplemented medium.

8. Putative mediators of insulin action: regulation of pyruvate dehydrogenase and adenylate cyclase activities.

9. Influence of cyclic 3',5'-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells.

10. Role of cyclic 3':5'-adenosine monophosphate in the early transport changes induced by serum and insulin in quiescent fibroblasts.