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Literature DB >> 4351171

Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.

Abstract

A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.

Entities: Chemical Disease Species

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Year: 1973 PMID： 4351171 PMCID： PMC433487 DOI： 10.1073/pnas.70.5.1316

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

7 in total

1. Isolation of an electrophoretically homogeneous nonhistone nucleolar protein.

Authors: M E Knecht; H Busch
Journal: Life Sci II Date: 1971-11-22

2. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.

Authors: C S Teng; C T Teng; V G Allfrey
Journal: J Biol Chem Date: 1971-06-10 Impact factor: 5.157

3. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.

Authors: E Kaltschmidt; H G Wittmann
Journal: Anal Biochem Date: 1970-08 Impact factor: 3.365

4. Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.

Authors: C C Sherton; I G Wool
Journal: J Biol Chem Date: 1972-07-25 Impact factor: 5.157

5. Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate.

Authors: R Balhorn; M Balhorn; H P Morris; R Chalkley
Journal: Cancer Res Date: 1972-08 Impact factor: 12.701

6. Enumeration of rabbit reticulocyte ribosomal proteins.

Authors: O H Martini; H J Gould
Journal: J Mol Biol Date: 1971-12-14 Impact factor: 5.469

7. Limited heterogeneity of the major nonhistone chromosomal proteins.

Authors: S C Elgin; J Bonner
Journal: Biochemistry Date: 1970-10-27 Impact factor: 3.162

7 in total

63 in total

10. Nucleolin overexpression is associated with an unfavorable outcome for ependymoma: a multifactorial analysis of 176 patients.

Authors: Chunjui Chen; Lingchao Chen; Yu Yao; Zhiyong Qin; Hong Chen
Journal: J Neurooncol Date: 2015-11-28 Impact factor: 4.130

Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.

1. Isolation of an electrophoretically homogeneous nonhistone nucleolar protein.

2. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.

3. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.

4. Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.

5. Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate.

6. Enumeration of rabbit reticulocyte ribosomal proteins.

7. Limited heterogeneity of the major nonhistone chromosomal proteins.

Review 1. The relation of gene control mechanisms to cancer.

2. Chromosomal proteins in hepatocarcinogenesis.

3. Fine structure of active ribosomal genes.

4. Micro- and nanofluidic technologies for epigenetic profiling.

5. High resolution two-dimensional electrophoresis of proteins.

6. Two splice variants of Nopp140 in Drosophila melanogaster.

7. Nucleolin provides a link between RNA polymerase I transcription and pre-ribosome assembly.

8. The 25-kDa FK506-binding protein is localized in the nucleus and associates with casein kinase II and nucleolin.

9. Nucleolin: acharan sulfate-binding protein on the surface of cancer cells.

10. Nucleolin overexpression is associated with an unfavorable outcome for ependymoma: a multifactorial analysis of 176 patients.