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Literature DB >> 4349185

Production and purification of large amounts of Rous sarcoma virus.

Abstract

Procedures are described for production and purification of large amounts of Rous sarcoma virus. The virus was produced by Rous sarcoma virus-transformed chicken embryo fibroblasts in roller culture which produced up to 6 mg of virus per day per liter of supernatant fluid. Various methods of concentrating virus were evaluated; pelleting yielded the best results in terms of recovery of infectious virus. Purification was achieved by means of successive velocity and equilibrium density centrifugation by using sucrose solutions made in low-salt buffer. A rapid method for the optical density measurement of virus concentration was also developed.

Entities: Chemical Species

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Year: 1973 PMID： 4349185 PMCID： PMC380809 DOI： 10.1128/am.25.3.346-353.1973

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

28 in total

1. Polypeptide components of virions, top component and cores of reovirus type 3.

Authors: R E Smith; H J Zweerink; W K Joklik
Journal: Virology Date: 1969-12 Impact factor: 3.616

2. The oncogenic effects of nontransforming viruses from avian myeloblastosis virus.

Authors: R E Smith; C Moscovici
Journal: Cancer Res Date: 1969-07 Impact factor: 12.701

3. Characteristics of two new avian tumor virus subgroups.

Authors: R G Duff; P K Vogt
Journal: Virology Date: 1969-09 Impact factor: 3.616

4. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions.

Authors: K Toyoshima; P K Vogt
Journal: Virology Date: 1969-07 Impact factor: 3.616

5. Effects of genetic cellular resistance on cell transformation and virus replication in chicken hematopoietic cell cultures infected with avian myeloblastosis virus (BAI-A).

Authors: C Moscovici; P K Vogt
Journal: Virology Date: 1968-08 Impact factor: 3.616

6. Proteins of Rous sarcoma virus.

Authors: P H Duesberg; H L Robinson; W S Robinson; R J Huebner; H C Turner
Journal: Virology Date: 1968-09 Impact factor: 3.616

7. A method for the propagation of large amounts of Rous sarcoma virus.

Authors: J P Bader
Journal: Virology Date: 1968-09 Impact factor: 3.616

8. A quantitative assay for avian myeloblastosis virus.

Authors: C Moscovici
Journal: Proc Soc Exp Biol Med Date: 1967 Aug-Sep

9. Relationship between DNA synthesis and cell density in normal and virus-transformed cells.

Authors: A Macieira-Coelho
Journal: Int J Cancer Date: 1967-07-15 Impact factor: 7.396

10. Studies on carcinogenesis by avian sarcoma viruses. 3. The differential effect of serum and polyanions on multiplication of uninfected and converted cells.

Authors: H M Temin
Journal: J Natl Cancer Inst Date: 1966-08 Impact factor: 13.506

22 in total

1. Infectivity of proviral DNA from avian sarcoma virus-transformed mammalian cells.

Authors: F Catala; P Vigier
Journal: J Virol Date: 1979-03 Impact factor: 5.103

2. Effect of viral RNase H on the avian sarcoma viral genome during early transcription in vitro.

Authors: R Friedrich; K Moelling
Journal: J Virol Date: 1979-09 Impact factor: 5.103

3. Recovery of murine leukemia virus from large volumes of freshly harvested culture fluids by using a single density gradient.

Authors: P Sottong; P Hill; M Feeney; J Klecker; K Johnson; R Harris; C Bell; K Stafford
Journal: Appl Microbiol Date: 1975-01

4. Calcium-dependent regulatory protein of cyclic nucleotide metabolism in normal and transformed chicken embryo fibroblasts.

Authors: D M Watterson; L J Van Eldik; R E Smith; T C Vanaman
Journal: Proc Natl Acad Sci U S A Date: 1976-08 Impact factor: 11.205

5. Molecular weight of two oncornavirus genomes: derivation from particle molecular weights and RNA content.

Authors: A R Bellamy; S C Gillies; J D Harvey
Journal: J Virol Date: 1974-12 Impact factor: 5.103

6. Adaptation and infection of mouse bone marrow (JLS-V9) cells in suspension culture for production of Rauscher leukemia virus.

Authors: H M Hodge; F Klein; A K Bandyopadhyay; O R Robinson; G P Shibley
Journal: Appl Microbiol Date: 1974-01

7. Sequences from myeloblastosis-associated virus MAV-2(O) and UR2AV involved in the formation of plaques and the induction of osteopetrosis, anemia, and ataxia.

Authors: R E Aurigemma; J L Torgersen; R E Smith
Journal: J Virol Date: 1991-01 Impact factor: 5.103

8. Bleomycin: action on growth of oncogenic RNA viruses and on cell transformation.

Authors:
Journal: Arch Virol Date: 1975 Impact factor: 2.574

9. Correlation of RNA binding affinity of avian oncornavirus p19 proteins with the extent of processing of virus genome RNA in cells.

Authors: J P Leis; P Scheible; R E Smith
Journal: J Virol Date: 1980-09 Impact factor: 5.103

10. Purification of infectious feline leukemia virus from large volumes of tissue culture fluids.

Authors: L E Mathes; D S Yohn; R G Olsen
Journal: J Clin Microbiol Date: 1977-03 Impact factor: 5.948