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Literature DB >> 4344958

Simplified radioimmunoassay for diagnostic serology.

Abstract

A simplified, indirect radioimmunoassay is described for Escherichia coli, vaccinia virus, and herpesvirus. The antigens were affixed to glass cover slips; thus both the primary and secondary reactions take place on the cover slips, and the unbound antiserum is easily separated from the bound antiserum by rinsing. Rabbit or human immune sera were reacted with the antigens, and the primary immune complex was quantitated by a secondary reaction with (125)I-indicator globulin (anti-rabbit or anti-human). A direct relationship between the antiserum concentration and the (125)I absorption was established. Variations in titers were detectable, and the titers were comparable to complement fixation titers. Homologous and heterologous reactions were distinguishable. The method affords an objective, quantitative, and qualitative evaluation of antibody, and results are reproducible.

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Year: 1972 PMID： 4344958 PMCID： PMC380656 DOI： 10.1128/am.24.5.742-749.1972

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

12 in total

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors: F C GREENWOOD; W M HUNTER; J S GLOVER
Journal: Biochem J Date: 1963-10 Impact factor: 3.857

2. Precipitation of radiolabeled poliovirus with specific antibody and antiglobulin.

Authors: R K GERLOFF; B H HOYER; L C McLAREN
Journal: J Immunol Date: 1962-10 Impact factor: 5.422

3. The serum proteins in multiple myelomatosis.

Authors: R A Kekwick
Journal: Biochem J Date: 1940-09 Impact factor: 3.857

4. A method of trace iodination of proteins for immunologic studies.

Authors: P J McConahey; F J Dixon
Journal: Int Arch Allergy Appl Immunol Date: 1966

5. Immunofluorescence in cells derived from Burkitt's lymphoma.

Authors: G Henle; W Henle
Journal: J Bacteriol Date: 1966-03 Impact factor: 3.490

6. Application of the paired radioiodine-labeled antibody technique (PRILAT) to the detection of adenovirus 12 tumor (T) antigen.

Authors: M J Evans; D S Yohn
Journal: J Immunol Date: 1970-05 Impact factor: 5.422

Review 7. General principles of radioimmunoassay.

Authors: S A Berson; R S Yalow
Journal: Clin Chim Acta Date: 1968-09 Impact factor: 3.786

8. Frequency of antibody to hepatitis-associated antigen as measured by a new radioimmunoassay technique.

Authors: J J Lander; H J Alter; R H Purcell
Journal: J Immunol Date: 1971-05 Impact factor: 5.422

9. Automation of an indirect fluorescent antibody test for syphilis.

Authors: G W Stout; J S Lewis
Journal: Ann N Y Acad Sci Date: 1971-06-21 Impact factor: 5.691

10. An approach toward automation of antinuclear antibody (ANA) determination.

Authors: J H ten Veen; A C Kuivenhoven; T E Feltkamp
Journal: Ann N Y Acad Sci Date: 1971-06-21 Impact factor: 5.691

12 in total

1. Detection of virus-specific immunoglobulins using a doubly labeled fluorescein-125I antibody.

Authors: A J Parkinson; J Kalmakoff
Journal: J Clin Microbiol Date: 1976-06 Impact factor: 5.948

2. Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.

Authors: K O Kalimo; O H Meurman; P E Halonen; B R Ziola; M K Viljanen; K Granfors; P Toivanen
Journal: J Clin Microbiol Date: 1976-08 Impact factor: 5.948

3. Screening for mumps immunity with the microtiter solid-phase radioimmunoassay.

Authors: R Hernandez; M Just; A Bürgin-Wolff
Journal: Infection Date: 1976 Impact factor: 3.553

Review 4. Review of bovine immunology for the veterinary practitioner.

Authors: B N Wilkie
Journal: Can Vet J Date: 1974-09 Impact factor: 1.008

5. Radioimmunoassay on polycarbonate membranes: a sensitive and simplified method for the detection and quantitation of antibody.

Authors: R L Green; R W Scales; S J Kraus
Journal: Appl Microbiol Date: 1974-03

9. Comparison of rabies humoral antibody titers in rabbits and humans by indirect radioimmunoassay, rapid-fluorescent-focus-inhibition technique, and indirect fluorescent-antibody assay.

Authors: T K Lee; H D Hutchinson; D W Ziegler
Journal: J Clin Microbiol Date: 1977-03 Impact factor: 5.948

10. Local antibody response in chickens: analysis of antibody synthesis to Newcastle disease virus by solid-phase radioimmunoassay and immunofluorescence with class-specific antibody for chicken immunoglobulins.

Authors: D L Ewert; B O Barger; C S Eidson
Journal: Infect Immun Date: 1979-04 Impact factor: 3.441