Literature DB >> 4340749

Loss of simian virus 40 DNA-RNA hybrids from nitrocellulose membranes; implications for the study of virus--host DNA interactions.

M Haas, M Vogt, R Dulbecco.   

Abstract

Complete hybrids of simian virus 40 (SV40)DNA and its complementary RNA (cRNA) are not retained on nitrocellulose membranes. At saturating cRNA concentrations, retention of the hybrids indicates incomplete homology between DNA and RNA, probably due to incorporation of host DNA in the viral DNA; this effect is most pronounced when DNA is produced in cells infected at high multiplicity. Hybrids between DNA of Chinese hamster cells transformed by SV40 and cRNA are retained if the DNA fragments are long, but they are lost if the DNA is sheared to less than the length of an SV40 DNA molecule. Hence, in cells examined with about six SV40 genomes per cell, each genome is individually integrated. The results may explain previous discrepancies in the estimation of the number of viral genomes in transformed cells.

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Year:  1972        PMID： 4340749      PMCID： PMC426891          DOI： 10.1073/pnas.69.8.2160

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes.

Authors:  L D Gelb; D E Kohne; M A Martin
Journal:  J Mol Biol       Date:  1971-04-14       Impact factor: 5.469

2.  SV40 DNA strand selection by Escherichia coli RNA polymerase.

Authors:  H Westphal
Journal:  J Mol Biol       Date:  1970-06-14       Impact factor: 5.469

3.  Viral DNA in polyoma- and SV40-transformed cell lines.

Authors:  H Westphal; R Dulbecco
Journal:  Proc Natl Acad Sci U S A       Date:  1968-04       Impact factor: 11.205

4.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

5.  Clustered genes for ribosomal ribonucleic acids in Escherichia coli.

Authors:  S Spadari; F Ritossa
Journal:  J Mol Biol       Date:  1970-11-14       Impact factor: 5.469

6.  A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors:  R R Burgess
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

7.  A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.

Authors:  D Gillespie; S Spiegelman
Journal:  J Mol Biol       Date:  1965-07       Impact factor: 5.469

8.  Denaturation of RNA with dimethyl sulfoxide.

Authors:  J H Strauss; R B Kelly; R L Sinsheimer
Journal:  Biopolymers       Date:  1968-06       Impact factor: 2.505

9.  Molecular hybridization between rat liver deoxyribonucleic acid and complementary ribonucleic acid.

Authors:  M Melli; J O Bishop
Journal:  Biochem J       Date:  1970-11       Impact factor: 3.857

10.  The ribonuclease activity of crystallized pancreatic deoxyribonuclease.

Authors:  S B Zimmerman; D Sandeen
Journal:  Anal Biochem       Date:  1966-02       Impact factor: 3.365
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  10 in total

1.  Transcriptional complexity of vaccinia virus in vivo and in vitro.

Authors:  E Paoletti; L J Grady
Journal:  J Virol       Date:  1977-09       Impact factor: 5.103

2.  Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.

Authors:  G R Stark; J G Williams
Journal:  Nucleic Acids Res       Date:  1979-01       Impact factor: 16.971

3.  Quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with Rous sarcoma virus.

Authors:  J T Parsons; J M Coffin; R K Haroz; P A Bromley; C Weissmann
Journal:  J Virol       Date:  1973-05       Impact factor: 5.103

4.  Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

Authors:  J Brandsma; G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

5.  Groups of adenovirus type 2 mRNA's derived from a large primary transcript: probable nuclear origin and possible common 3' ends.

Authors:  J R Nevins; J E Darnell
Journal:  J Virol       Date:  1978-03       Impact factor: 5.103

6.  Analysis of simian virus 40 DNA with the restriction enzyme of Haemophilus aegyptius, endonuclease Z.

Authors:  E S Huang; J E Newbold; J S Pagano
Journal:  J Virol       Date:  1973-04       Impact factor: 5.103

7.  Interaction of polyoma and mouse DNAs. IV. Time course and extent of integration of polyoma DNA into mouse DNA during lytic infection.

Authors:  H Türler
Journal:  J Virol       Date:  1977-08       Impact factor: 5.103

8.  Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IX. Template topography in the early region of simian virus 40.

Authors:  C T Patch; A M Lewis; A S Levine
Journal:  J Virol       Date:  1974-03       Impact factor: 5.103

9.  Deoxyribonucleic acid of Marek's disease virus in virus-induced tumors.

Authors:  K Nazerian; T Lindahl; G Klein; L F Lee
Journal:  J Virol       Date:  1973-10       Impact factor: 5.103

10.  Titration of integrated simian virus 40 DNA sequences, using highly radioactive, single-stranded DNA probes.

Authors:  M A Marchionni; D J Roufa
Journal:  J Virol       Date:  1981-04       Impact factor: 5.103
  10 in total

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