Literature DB >> 4339196

Properties of somatic cell hybrids between mouse cells and simian virus 40-transformed rat cells.

J van der Noordaa, A van Haagen, J M Walboomers, H van Someren.   

Abstract

Hybrids between mouse cells and simian virus 40 (SV40)-transformed rat cells were made, and their properties and chromosome constitution were investigated over many generations. Their hybrid nature was confirmed by enzyme studies. During a period of 1 year a loss of 10 to 20% of the total number of chromosomes was observed. The SV40 tumor antigen was present and remained present in the hybrids. The parental and hybrid cells were studied for agglutination with concanavalin A, for growth in soft agar, and for serum requirement. These growth and surface characteristics of the transformed cells appeared in the hybrids.

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Year:  1972        PMID： 4339196      PMCID： PMC356426     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  12 in total

1.  SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.

Authors:  J W LITTLEFIELD
Journal:  Science       Date:  1964-08-14       Impact factor: 47.728

2.  Properties of hybrids between polyoma-transformed and normal mouse cells.

Authors:  V Defendi; B Ephrussi; H Koprowski; M C Yoshida
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

3.  Studies of Interspecific (Rat x Mouse) Somatic Hybrids. I. Isolation, Growth and Evolution of the Karyotype.

Authors:  M C Weiss; B Ephrussi
Journal:  Genetics       Date:  1966-11       Impact factor: 4.562

4.  Enzyme electrophoresis on cellulose acetate gel: zymogram patterns in mgh-mouse and man--Chinese hamster somatic cell hybrids.

Authors:  P Meera Khan
Journal:  Arch Biochem Biophys       Date:  1971-08       Impact factor: 4.013

5.  Binding of radioactively labelled concanavalin A and wheat germ agglutinin to normal and virus-transformed cells.

Authors:  B Ozanne; J Sambrook
Journal:  Nat New Biol       Date:  1971-08-04

6.  Further studies on loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells.

Authors:  M C Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  1970-05       Impact factor: 11.205

7.  Selection of morphologically normal cell lines from polyoma-transformed BHK21/13 hamster fibroblasts.

Authors:  G Marin; J W Littlefield
Journal:  J Virol       Date:  1968-01       Impact factor: 5.103

8.  Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propiolactone-inactivated Sendai virus.

Authors:  J M Neff; J F Enders
Journal:  Proc Soc Exp Biol Med       Date:  1968-01

9.  Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells.

Authors:  M Inbar; L Sachs
Journal:  Proc Natl Acad Sci U S A       Date:  1969-08       Impact factor: 11.205

10.  Growth characteristics of virus-transformed cells. Maximum population density, inhibition by normal cells, serum requirement, growth in soft agar, and xenogeneic transplantability.

Authors:  H Eagle; G E Foley; H Koprowski; H Lazarus; E M Levine; R A Adams
Journal:  J Exp Med       Date:  1970-04-01       Impact factor: 14.307
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  2 in total

Review 1.  Utilization of somatic cell hybrids for genetic studies in man.

Authors:  K H Grzeschik
Journal:  Humangenetik       Date:  1973

2.  Ultrastructural localization of concanavalin A receptors in somatic cell hybrids between normal mouse cells and simian virus 40-transformed rat cells.

Authors:  C Wallig; J M Walboomers; J van der Noordaa
Journal:  J Cell Biol       Date:  1974-05       Impact factor: 10.539
  2 in total

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