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Literature DB >> 4325022

Semi-micro, dye-binding assay for rabbit interferon.

Abstract

The semi-micro method for interferon assay was based on quantitation of inhibition of cytopathic effects in 6-mm wells. Cultures were exposed to interferon dilutions, challenged with vesicular stomatitis virus, and stained with methylrosanaline chloride. The bound dye was then eluted and measured colorimetrically. The method was more sensitive than the plaque-inhibition method and was extremely economical. Culture age was the most important factor affecting reproductibility.

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Year: 1971 PMID： 4325022 PMCID： PMC377263 DOI： 10.1128/am.21.4.723-725.1971

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

2 in total

1. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon.

Authors: J LINDENMANN; G E GIFFORD
Journal: Virology Date: 1963-03 Impact factor: 3.616

2. Microassay for human and chick cell interferons.

Authors: J G Tilles; M Finland
Journal: Appl Microbiol Date: 1968-11

2 in total

107 in total

1. Optimization of cell culture conditions for production of biologically active proteins.

Authors: S Hosoi; H Miyaji; M Satoh; T Kurimoto; A Mihara; N Fujiyoshi; S Itoh; S Sato
Journal: Cytotechnology Date: 1991-01 Impact factor: 2.058

2. T-antigen expression in human skin fibroblasts is not regulated by an endogenous interferon response to SV40 infection.

Authors: A S Lubiniecki; W A Blattner; V Cruttenden; M Gunnell; G C Tarr; J F Fraumeni
Journal: Arch Virol Date: 1978 Impact factor: 2.574

7. Spontaneous production of human gamma interferon in vitro by splenic lymphocytes of patients with idiopathic thrombocytopenic purpura.

Authors: Y W Hu; G X Xue; Y H Tan
Journal: Clin Exp Immunol Date: 1984-05 Impact factor: 4.330

Semi-micro, dye-binding assay for rabbit interferon.

1. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon.

2. Microassay for human and chick cell interferons.

1. Optimization of cell culture conditions for production of biologically active proteins.

2. T-antigen expression in human skin fibroblasts is not regulated by an endogenous interferon response to SV40 infection.

3. Protective effect of early serum from mice after cytomegalovirus infection.

4. Specificity of interferon action in protein synthesis.

5. Cell-free synthesis of human interferon.

6. Multisurface glass roller bottle for growth of animal cells in culture.

7. Spontaneous production of human gamma interferon in vitro by splenic lymphocytes of patients with idiopathic thrombocytopenic purpura.

8. Cellular alteration by interferon: a virus-free system for assaying interferon.

9. Control of interferon synthesis: effect of diethylaminoethyl-dextran on induction by polyinosinic-polycytidylic acid.

10. Interferon induction in rabbit cells irradiated with UV light.