Literature DB >> 4325021

Production and evaluation of a purified adenovirus group-specific (hexon) antigen for use in the diagnostic complement fixation test.

W R Dowdle, M Lambriex, J C Hierholzer.   

Abstract

A simple procedure for the production of large volumes of purified adenovirus group-specific complement-fixing (CF) (hexon) antigen by selective adsorption to and elution from CaHPO(4) is described. Results of immunodiffusion tests, electrophoresis, electron microscopy, and tests for hemagglutination and infectivity indicate that the purified antigen consisted of a single virus component (hexon). The purified product contained little host materials. Unlike the crude virus harvest usually employed for serodiagnostic CF tests, the purified antigen demonstrated no anticomplementary activity and did not develop such activity during storage. The purified antigen was equal to or slightly more sensitive than crude virus harvests for serodiagnosis of adenovirus infections.

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Year:  1971        PMID： 4325021      PMCID： PMC377262          DOI： 10.1128/am.21.4.718-722.1971

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  12 in total

1.  Chromatography of adenoviruses on calcium phosphate columns.

Authors:  M SIMON
Journal:  Acta Virol       Date:  1962-07       Impact factor: 1.162

2.  Identification and classification of adenoviruses.

Authors:  H S GINSBERG
Journal:  Virology       Date:  1962-10       Impact factor: 3.616

3.  The purification and concentration of viruses and virus soluble antigens on calcium phosphate.

Authors:  J TAVERNE; J H MARSHALL; F FULTON
Journal:  J Gen Microbiol       Date:  1958-12

4.  Hemagglutination-inhibition antibody responses in human adenovirus infections.

Authors:  L ROSEN
Journal:  Proc Soc Exp Biol Med       Date:  1961-11

5.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

6.  Neutralizing, hemagglutination-inhibiting and group complement-fixing antibody responses in human adenovirus infections.

Authors:  N J Schmidt; E H Lennette; C J King
Journal:  J Immunol       Date:  1966-07       Impact factor: 5.422

Review 7.  The structural and functional diversity of Adenovirus capsid components.

Authors:  E Norrby
Journal:  J Gen Virol       Date:  1969-09       Impact factor: 3.891

8.  Immunological basis of the adenovirus 8-9 cross-reaction.

Authors:  J C Hierholzer; W R Dowdle
Journal:  J Virol       Date:  1970-12       Impact factor: 5.103

9.  Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation.

Authors:  J C Hierholzer; M T Suggs; E C Hall
Journal:  Appl Microbiol       Date:  1969-11

10.  The sensitivity of the complement fixation test for the detection of adenovirus infections in infants and children with lower respiratory disease.

Authors:  B Portnoy; M A Salvatore; B Hanes; D I Hammer; J M Leedom; A Jambazian; H L Eckert
Journal:  Am J Epidemiol       Date:  1967-09       Impact factor: 4.897
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  10 in total

1.  New human adenovirus associated with respiratory illness: candidate adenovirus type 39.

Authors:  J C Hierholzer; M C Kemp; G W Gary; H C Spencer
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

2.  Reference equine antisera to 33 human adenovirus types: homologous and heterologous titers.

Authors:  J C Hierholzer; W C Gamble; W R Dowdle
Journal:  J Clin Microbiol       Date:  1975-01       Impact factor: 5.948

3.  New human adenovirus isolated from a renal transplant recipient: description and characterization of candiate adenovirus type 34.

Authors:  J C Hierholzer; N O Atuk; J M Gwaltney
Journal:  J Clin Microbiol       Date:  1975-04       Impact factor: 5.948

4.  Antigenic characterization of intermediate adenovirus 14-11 strains associated with upper respiratory illness in a military camp.

Authors:  J C Hierholzer; A Pumarola
Journal:  Infect Immun       Date:  1976-02       Impact factor: 3.441

5.  Application of a solid-phase immunofluorometric assay to the selection of monoclonal antibody specific for the adenovirus group-reactive hexon antigen.

Authors:  J C Hierholzer; D J Phillips; D D Humphrey; R A Coombs; C B Reimer
Journal:  Arch Virol       Date:  1984       Impact factor: 2.574

6.  Characteristics of noncultivable adenoviruses associated with diarrhea in infants: a new subgroup of human adenoviruses.

Authors:  G W Gary; J C Hierholzer; R E Black
Journal:  J Clin Microbiol       Date:  1979-07       Impact factor: 5.948

7.  Comparison of immunization methods for producing reference adenovirus antisera in horses.

Authors:  J C Hierholzer; W C Gamble; K D Quist; W A Chappell
Journal:  Appl Microbiol       Date:  1972-09

8.  Immunoassay diagnosis of adenovirus infections in children.

Authors:  O Meurman; O Ruuskanen; H Sarkkinen
Journal:  J Clin Microbiol       Date:  1983-11       Impact factor: 5.948

9.  New human adenovirus (candidate adenovirus type 35) causing fatal disseminated infection in a renal transplant recipient.

Authors:  H Stalder; J C Hierholzer; M N Oxman
Journal:  J Clin Microbiol       Date:  1977-09       Impact factor: 5.948

10.  Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection.

Authors:  Inarei Paulini; Joselma Siqueira-Silva; Luciana Thomaz; Leticia Rocha; Charlotte Harsi; Nancy Bellei; Celso Granato
Journal:  Braz J Infect Dis       Date:  2017-06-15       Impact factor: 3.257
  10 in total

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