Effects of amiloride on active sodium transport by the isolated frog skin: evidence concerning site of action.

1. Amiloride reduces short-circuit current and potential difference across the isolated frog skin.2. Isotopically measured sodium influx and efflux are diminished.3. Total electrical conductance and partial sodium conductance are diminished, the reduction in total conductance being entirely accounted for by the reduction in partial sodium conductance.4. The effect of antidiuretic hormone (ADH), cyclic 3'5'-adenosine monophosphate (cyclic AMP) and theophylline can be antagonized by pretreatment with amiloride but the antagonism can be abolished by increasing the concentration of these compounds.5. Amiloride has no effect on oxygen consumption in concentrations which inhibit sodium transport. However, it prevents the stimulatory effect of ADH on oxygen consumption.6. The results are consistent with an action of amiloride at the passive outside membrane of the transporting cells of isolated frog skin.