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Literature DB >> 4309311

1-Anilinonaphthalene-8-sulphonate, a fluorescent conformational probe for glutamate dehydrogenase.

Abstract

1. The interaction of 1-anilinonaphthalene-8-sulphonate with ox liver glutamate dehydrogenase was examined. 2. The fluorescence of the dye is enhanced 100-fold on binding. 3. A further enhancement is observed when NADH and GTP are added to the enzyme. 4. By using this property of the dye to measure conformational equilibria in the enzyme the effects of coenzyme, inhibitors, enzyme concentration, ionic strength and pH on the allosteric transitions were studied. 5. GTP and NADH interact with the enzyme in a heterotropic manner. 6. The rate of the structural transition brought about by GTP and NADH is biphasic with half-lives of 34 and 200msec. 7. The relation of these observations to regulatory mechanisms is discussed.

Entities: Chemical

Mesh：

Substances：

Year: 1969 PMID： 4309311 PMCID： PMC1184868 DOI： 10.1042/bj1140407

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

23 in total

1. Desensitization of the allosteric sites of glutamate dehydrogenase by fluorodinitrobenzene.

Authors: G Di Prisco
Journal: Biochem Biophys Res Commun Date: 1967-01-23 Impact factor: 3.575

2. Kinetic studies of dogfish liver glutamate dehydrogenase with diphosphopyridine nucleotide and the effect of added salts.

Authors: L Corman; N O Kaplan
Journal: J Biol Chem Date: 1967-06-25 Impact factor: 5.157

3. Conformational changes and the regulation of glutamate-dehydrogenase activity.

Authors: P M Bayley; G K Radda
Journal: Biochem J Date: 1966-01 Impact factor: 3.857

4. On the role of amino groups in the structure and function of glutamate dehydrogenase. I. Effect of acetylation on catalytic and regulatory properties.

Authors: R F Colman; C Frieden
Journal: J Biol Chem Date: 1966-08-25 Impact factor: 5.157

5. On the role of amino groups in the structure and function of glutamate dehydrogenase. II. Effect of acetylation on molecular properties.

Authors: R F Colman; C Frieden
Journal: J Biol Chem Date: 1966-08-25 Impact factor: 5.157

9. The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites.

Authors: L Stryer
Journal: J Mol Biol Date: 1965-09 Impact factor: 5.469

10. Inhibition of glutamic dehydrogenase by pyridoxal 5'-phosphate.

Authors: B M Anderson; C D Anderson; J E Churchich
Journal: Biochemistry Date: 1966-09 Impact factor: 3.162

13 in total

1. Conformational studies on phospholipase C from Bacillus cereus. The effect of urea on the enzyme.

Authors: C Little
Journal: Biochem J Date: 1978-12-01 Impact factor: 3.857

2. Ion association reactions with biological membranes, studied with the fluorescent dye 1-anilino-8-naphthalenesulfonate.

Authors: B Gomperts; F Lantelme; R Stock
Journal: J Membr Biol Date: 1970-12 Impact factor: 1.843

9. Characterization of cell subpopulations of the thymus by a hydrophobic fluorescent probe: 1-anilino-8-naphthalene sulphonate.

Authors: J Vaillier; D Vaillier
Journal: Clin Exp Immunol Date: 1977-11 Impact factor: 4.330

10. Desensitization of glutamate dehydrogenase by reaction of tyrosne residues.

Authors: N C Price; G K Radda
Journal: Biochem J Date: 1969-09 Impact factor: 3.857

1-Anilinonaphthalene-8-sulphonate, a fluorescent conformational probe for glutamate dehydrogenase.

1. Desensitization of the allosteric sites of glutamate dehydrogenase by fluorodinitrobenzene.

2. Kinetic studies of dogfish liver glutamate dehydrogenase with diphosphopyridine nucleotide and the effect of added salts.

3. Conformational changes and the regulation of glutamate-dehydrogenase activity.

4. On the role of amino groups in the structure and function of glutamate dehydrogenase. I. Effect of acetylation on catalytic and regulatory properties.

5. On the role of amino groups in the structure and function of glutamate dehydrogenase. II. Effect of acetylation on molecular properties.

6. Apparatus for rapid and sensitive spectrophotometry.

7. On the nature of allosteric transitions: implications of non-exclusive ligand binding.

8. Comparison of experimental binding data and theoretical models in proteins containing subunits.

9. The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites.

10. Inhibition of glutamic dehydrogenase by pyridoxal 5'-phosphate.

1. Conformational studies on phospholipase C from Bacillus cereus. The effect of urea on the enzyme.

2. Ion association reactions with biological membranes, studied with the fluorescent dye 1-anilino-8-naphthalenesulfonate.

3. Thiol groups of ribonucleic acid polymerase.

4. The chemistry of flavins and flavoproteins: aerobic photochemistry.

5. The binding of oxidized coenzymes by glutamate dehydrogenase and the effects of glutarate and purine nucleotides.

6. Chemical modification of glutamate dehydrogenase by 2,4,6-trinitrobenzenesulphonic acid.

7. Interaction of 1-anilino-8-naphthalene sulfonate with yeast glyceraldehyde-3-phosphate dehydrogenase.

8. A chemical-modification approach to the olfactory code. Studies with a thiol-specific reagent.

9. Characterization of cell subpopulations of the thymus by a hydrophobic fluorescent probe: 1-anilino-8-naphthalene sulphonate.

10. Desensitization of glutamate dehydrogenase by reaction of tyrosne residues.