Conjugation of androsterone by the guinea-pig kidney in vitro.

Radioactive androsterone was incubated with kidney slices from guinea pigs in a Ringer bicarbonate buffer. For analysis, the radioactivity was partitioned between chloroform and water for the separation of non-polar and polar steroids. After multiple chromatography procedures, the non-polar fraction was shown to contain androsterone and at least four other similar steroids, not identified in this study. The polar fraction yielded numerous conjugates from which androsterone glucuronide and androsterone sulphate in approximately equal quantities were isolated and characterized.