Literature DB >> 43056

[Simple method for the preparation of haptoglobin].

W Schössler, H Zorn, D Patzelt.   

Abstract

The present paper deals with a method permitting the isolation of haptoglobin 2-2 from human serum or plasma. The haptoglobin is adsorbed to DEAE-cellulose at pH 5.1 by batching. The loaded cellulose is given into a column and the haptoglobin eluted by a 0.1 M to 0.15 M acetate buffer. The last step is a gel-chromatography on Sephadex G-200. Disc- and immunoelectrophoresis were used to test the purity. As by-product acid alpha1-glycoprotein can be obtained.

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Year:  1979        PMID: 43056

Source DB:  PubMed          Journal:  Acta Biol Med Ger        ISSN: 0001-5318


  1 in total

1.  Haptoglobin subtypes in Berlin, GDR. A simple procedure for haptoglobin purification and subtyping.

Authors:  D Patzelt; H Schröder
Journal:  Z Rechtsmed       Date:  1985
  1 in total

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