| Literature DB >> 4296325 |
Abstract
1. A method of stabilizing the enzyme by using glycerol is described. 2. A purification procedure is presented giving a higher purification than previously described. 3. Data showing substrate activation and activation by citrate are presented. 4. Kinetic constants for NAD(+), NADH and certain bivalent metal ions are given. 5. Pronounced inhibitory buffer effects are described. 6. A brief comparison between the NAD-specific isocitrate dehydrogenase from peas and that from other sources is made.Entities:
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Year: 1967 PMID: 4296325 PMCID: PMC1198371 DOI: 10.1042/bj1050729
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857