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Literature DB >> 429214

Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased.

Abstract

Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4 degrees C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 mu, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 mu. The simultaneous coupling azo dye method using the substrates sodium alpha-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.

Entities: Chemical Species

Mesh：

Substances：
Alkaline Phosphatase

Year: 1979 PMID： 429214 DOI： 10.1007/bf00495731

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

7 in total

1. The distribution of certain enzyme systems in the normal human gastrointestinal tract.

Authors: I DAWSON; J PRYSE-DAVIES
Journal: Gastroenterology Date: 1963-06 Impact factor: 22.682

2. Histochemical comparison of naphthol AS-phosphates for the demonstration of phosphatases.

Authors: M S BURSTONE
Journal: J Natl Cancer Inst Date: 1958-03 Impact factor: 13.506

3. A simple method of preparing 1-2 microm sections of large tissue blocks using glycol methacrylate.

Authors: B Sims
Journal: J Microsc Date: 1974-07 Impact factor: 1.758

4. Adenocarcinoma, adenomas and polyps of the colon. Histochemical study.

Authors: B Czernobilsky; K C Tsou
Journal: Cancer Date: 1968-02 Impact factor: 6.860

5. Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains.

Authors: H S Bennett; A D Wyrick; S W Lee; J H McNeil
Journal: Stain Technol Date: 1976-03

6. The significance of enzyme histochemical patterns in carcinomas of the large intestine in man.

Authors: F McGinty; G Delides; D Harrison
Journal: Gut Date: 1973-06 Impact factor: 23.059

7. Freeze-drying and embedding in glycol methacrylate (GMA). The results of morphological, histochemical and immunohistological investigations.

Authors: D Mitrenga; W Arnold; H von Mayersbach
Journal: Histochemistry Date: 1974-06-05

7 in total

6 in total

1. Induction of mineralocorticoid receptor by sodium butyrate in small intestinal (IEC6) and colonic (T84) epithelial cell lines.

Authors: K Fukushima; I Sasaki; S Sato; H Sasano; Z Krozowski; S Matsuno
Journal: Dig Dis Sci Date: 1999-08 Impact factor: 3.199

Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased.

1. The distribution of certain enzyme systems in the normal human gastrointestinal tract.

2. Histochemical comparison of naphthol AS-phosphates for the demonstration of phosphatases.

3. A simple method of preparing 1-2 microm sections of large tissue blocks using glycol methacrylate.

4. Adenocarcinoma, adenomas and polyps of the colon. Histochemical study.

5. Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains.

6. The significance of enzyme histochemical patterns in carcinomas of the large intestine in man.

7. Freeze-drying and embedding in glycol methacrylate (GMA). The results of morphological, histochemical and immunohistological investigations.

1. Induction of mineralocorticoid receptor by sodium butyrate in small intestinal (IEC6) and colonic (T84) epithelial cell lines.

2. Sodium butyrate-induced liver-type alkaline phosphatase activity in a small intestinal epithelial cell line, IEC6.

3. Ultrastructural localisation of alkaline phosphatase in adult human large intestine.

Review 4. Butyrate and the colonocyte. Implications for neoplasia.

5. Peptide YY selectively stimulates expression of the colonocytic phenotype.

6. Serum free medium increases expression of markers of differentiation in human colonic crypt cells.