Characteristics of the photoreactivation of pseudorabies virus.

Pfefferkorn, E. R. (Harvard Medical School, Boston, Mass.), Boyce W. Burge, and Helen M. Coady. Characteristics of the photoreactivation of pseudorabies virus. J. Bacteriol. 92:856-861. 1966.-Pseudorabies virus, killed with ultraviolet light and then allowed to adsorb to chick embryo tissue culture cells, can be photoreactivated by light of longer wavelengths. The photoreactivable sector is approximately 0.5. Herpes simplex virus is equally photoreactivable. Photoreactivability is negligible during the first 20 min after adsorption and then rises to a maximum by 100 min. After this maximum is reached, photoreactivability decreases with increasing rate as the temperature of incubation is raised. Inhibition of protein synthesis immediately after absorption prevents the establishment of the photoreactivable state.