Respiration of mengovirus-infected L-929 cells.

Iglewski, W. J. (The Pennsylvania State University, University Park), and E. H. Ludwig. Respiration of mengovirus-infected L-929 cells. J. Bacteriol. 92:733-738. 1966.-Polarographic techniques were employed to study the oxidative metabolism of L-929 cells during a one-step mengovirus growth cycle. Virus maturation began 3.5 hr after infection and was complete with 7 hr. Virus maturation was accompanied by a decreased rate of endogenous respiration and an increased rate of oxidation of succinate and alpha-glycerophosphate by L-929 cells. The rate of glucose uptake was the same for mengovirus-infected and control L-929 cells. However, there was a decreased oxidation of glucose to carbon dioxide and a decreased production of lactic acid by L cells infected with mengovirus under aerobic conditions. Mengovirus was produced equally well under aerobic and anaerobic conditions. The implications of the alterations in metabolism with respect to virus synthesis are discussed.