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Literature DB >> 4284434

The preparation of rabbit renin-substrate for the assay of minute amounts of renin.

Abstract

1. By DEAE-cellulose chromatography and treatment with Cd(2+) a partially purified renin-substrate has been prepared from rabbit serum. 2. It has the following properties: (a) Angiotensinase activity is undetectable: 0.1mug. of angiotensin was recovered intact after incubation with the substrate at 37 degrees for 200hr. (b) It does not contain renin and does not generate vaso-active substances when incubated alone. (c) It is ten times as active as serum in terms of angiotensin produced/mg. of protein. (d) It has not deteriorated after storage for 3 months at 4 degrees at pH7.5.

Entities: Chemical Gene Species

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Year: 1965 PMID： 4284434 PMCID： PMC1207055 DOI： 10.1042/bj0960413

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

4 in total

1. A new method of large-scale preparation of hypertensin, with a note on its assay.

Authors: W S PEART
Journal: Biochem J Date: 1955-02 Impact factor: 3.857

2. Potentiometric determination of chloride in biological fluids.

Authors: P H SANDERSON
Journal: Biochem J Date: 1952-11 Impact factor: 3.857

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. The estimation of renin in plasma by an enzyme kinetic technique.

Authors: A F Lever; J I Robertson; M Tree
Journal: Biochem J Date: 1964-05 Impact factor: 3.857

4 in total

7 in total

1. [Reaction velocity of the enzymatic formation of angiotensin in vitro as a function of the electrolyte composition of the incubation medium].

Authors: H Dahlheim; P Weber; K Thurau
Journal: Pflugers Arch Date: 1969 Impact factor: 3.657

The preparation of rabbit renin-substrate for the assay of minute amounts of renin.

1. A new method of large-scale preparation of hypertensin, with a note on its assay.

2. Potentiometric determination of chloride in biological fluids.

3. Protein measurement with the Folin phenol reagent.

4. The estimation of renin in plasma by an enzyme kinetic technique.

1. [Reaction velocity of the enzymatic formation of angiotensin in vitro as a function of the electrolyte composition of the incubation medium].

2. [Purification and characterization of red cell angiotensinase].

Review 3. Renin and angiotensin. A survey of some aspects.

4. [Studies on preparative concentration possibilities of the renin substrate (angiotensinogen)].

5. Immunocytochemical localization of angiotensinogen in rat liver and kidney.

6. Properties of renin substrate in rabbit plasma with a note on its assay.

7. A rapid simple method for the assay of renin in rabbit plasma.