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Literature DB >> 4279763

Immunofluorescence plaque assay for African swine fever virus.

J Tessler, W R Hess, I C Pan, R Trautman.

Abstract

Suitably diluted cell culture adapted African swine fever virus preparations were inoculated on VERO cell monolayers and grown on coverslips. Gum tragacanth was used as an overlay. After three days incubation at 37 degrees C the infected cultures were fixed with acetone and stained with fluorescent antibody conjugate. Fluorescing plaques consisted of 20-30 infected cells. THREE STATISTICAL CRITERIA FOR A QUANTITATIVELY RELIABLE ASSAY WERE MET: the Poisson distribution for plaque counts, linearity of the relationship between the concentration of virus and the plaque count and reproducibility of replicate titrations. The method is suitable for counts up to at least 70 plaques per 5 cm(2) coverslip and computed titers are reproducible within 0.16 log units with a total of 300 plaques enumerated.

Entities: Chemical Species

Mesh：

Year: 1974 PMID： 4279763 PMCID： PMC1319849

Source DB: PubMed Journal: Can J Comp Med ISSN： 0008-4050

12 in total

Review 4. Attenuated African swine fever virus through serial passaging of viruses in cell culture: a brief review on the knowledge gathered during 60 years of research.

Authors: Xiaoyue Zhang; Zhenzhong Wang; Shengqiang Ge; Yuanyuan Zuo; Haodong Lu; Yan Lv; Naijun Han; Yumei Cai; Xiaodong Wu; Zhiliang Wang
Journal: Virus Genes Date: 2022-10-14 Impact factor: 2.198

5. Indirect Immunoperoxidase Test (IPT) for Detection of Antibodies Against African Swine Fever Virus (ASFV) on African Green Monkey Cell Lines (Vero, MS).

Authors: Gallardo Carmina; R Nieto; M Arias
Journal: Methods Mol Biol Date: 2022

5 in total

Immunofluorescence plaque assay for African swine fever virus.

1. Quantitative applications of fluorescent antibody technique to influenza-virus-infected cell cultures.

2. [On statistics of the plaque test].

3. Adenovirus assay by the fluorescent cell-counting procedure.

4. Diagnosis of African swine fever by immunofluorescence.

5. A new overlay for plaquing animal viruses.

6. Segregation of a nonhemadsorbing African swine fever virus in tissue culture.

7. Use of agar diffusion precipitation test in the diagnosis of African swine fever.

8. Plaque formation by African swine fever virus.

9. Glomerulonephritis in Aleutian disease of mink: histological and immunofluorescence studies.

10. Enumeration of cell-infecting particles of Newcastle disease virus by the fluorescent antibody technique.

1. Plaque formation by African swine fever virus in chick embryo fibroblasts in the absence of CO2 atmosphere.

2. Comparative titers of egg assay against immunofluorescent assay of Chlamydia psittaci.

3. A microtechnique for the titration for African swine fever virus.

Review 4. Attenuated African swine fever virus through serial passaging of viruses in cell culture: a brief review on the knowledge gathered during 60 years of research.

5. Indirect Immunoperoxidase Test (IPT) for Detection of Antibodies Against African Swine Fever Virus (ASFV) on African Green Monkey Cell Lines (Vero, MS).